ReviewAnnals of the American Thoracic Society2014
Directly sampling the lung of a young child with cystic fibrosis reveals diverse microbiota.
Review in Annals of the American Thoracic Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 70 citations in OpenAlex.
- Ralstonia pickettii as an emerging pediatric pathogen: a mini-review of current evidence.Frontiers in pediatrics · 2026Review
- Direct assessment of airway microbiota in primary ciliary dyskinesia end-stage lung disease.ERJ open research · 2025Article
- Changing profile of bacterial infection and microbiome in cystic fibrosis: when to use antibiotics in the era of CFTR-modulator therapy.European respiratory review : an official journal of the European Respiratory Society · 2024Review
- Ectopic colonization by oral bacteria as an emerging theme in health and disease.FEMS microbiology reviews · 2024Review
- Predictive modeling of antibiotic eradication therapy success for new-onset Pseudomonas aeruginosa pulmonary infections in children with cystic fibrosis.PLoS computational biology · 2023Article
- Article
- Commensal Bacteria in the Cystic Fibrosis Airway Microbiome ReduceFrontiers in cellular and infection microbiology · 2022Article
- Polymicrobial Interactions in the Cystic Fibrosis Airway Microbiome Impact the Antimicrobial Susceptibility ofAntibiotics (Basel, Switzerland) · 2021Review
- Article
- Changes in the Cystic Fibrosis Airway Microbiome in Response to CFTR Modulator Therapy.Frontiers in cellular and infection microbiology · 2021Review
- Microbial Community Composition in Explanted Cystic Fibrosis and Control Donor Lungs.Frontiers in cellular and infection microbiology · 2021Article
- Review
- Parallel Analysis of Cystic Fibrosis Sputum and Saliva Reveals Overlapping Communities and an Opportunity for Sample Decontamination.mSystems · 2020Article
- mSphere of Influence: a Community To Study Communities.mSphere · 2020Article
- Assessment of stability and fluctuations of cultured lower airway bacterial communities in people with cystic fibrosis.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2019Article
- Influence of the lung microbiome on antibiotic susceptibility of cystic fibrosis pathogens.European respiratory review : an official journal of the European Respiratory Society · 2019Review
- BAL Fluid Metaproteome in Acute Respiratory Failure.American journal of respiratory cell and molecular biology · 2018Article
- Cystic fibrosis respiratory microbiota: unraveling complexity to inform clinical practice.Expert review of respiratory medicine · 2018Review
- Smoking is associated with quantifiable differences in the human lung DNA virome and metabolome.Respiratory research · 2018Article
- Microbial diversity within the airway microbiome in chronic pediatric lung diseases.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
rationaleThe airways of people with cystic fibrosis (CF) are chronically infected with a variety of bacterial species. Although routine culture methods are usually used to diagnose these infections, culture-independent, DNA-based methods have identified many bacterial species in CF respiratory secretions that are not routinely cultured. Many prior culture-independent studies focused either on microbiota in explanted CF lungs, reflecting end-stage disease, or those in oropharyngeal swabs, which likely sample areas in addition to the lower airways. Therefore, it was unknown whether the lower airways of children with CF, well before end-stage but with symptomatic lung disease, truly contained diverse microbiota.
objectivesTo define the microbiota in the diseased lung tissue of a child who underwent lobectomy for severe, localized CF lung disease.
methodsAfter pathologic examination verified that this child's lung tissue reflected CF lung disease, we used bacterial ribosomal RNA gene pyrosequencing and computational phylogenetic analysis to identify the microbiota in serial sections of the tissue. MEASUREMENTS AND MAIN
resultsThis analysis identified diverse, and anatomically heterogeneous, bacterial populations in the lung tissue that contained both culturable and nonculturable species, including abundant Haemophilus, Ralstonia, and Propionibacterium species. Routine clinical cultures identified only Staphylococcus aureus, which represented only a small fraction of the microbiota found by sequencing. Microbiota analysis of an intraoperative oropharyngeal swab identified predominantly Streptococcus species. The oropharyngeal findings therefore represented the lung tissue microbiota poorly, in agreement with findings from earlier studies of oropharyngeal swabs in end-stage disease.
conclusionsThese results support the concept that diverse and spatially heterogeneous microbiota, not necessarily dominated by "traditional CF pathogens," are present in the airways of young, symptomatic children with early CF lung disease.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.