ArticleFrontiers in genetics2014
Using a medium-throughput comet assay to evaluate the global DNA methylation status of single cells.
Article in Frontiers in genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Measuring DNA modifications with the comet assay: a compendium of protocols.Nature protocols · 2023Review
- Bi-directional effects of vitamin BScientific reports · 2017Article
- The development and validation of EpiComet-Chip, a modified high-throughput comet assay for the assessment of DNA methylation status.Environmental and molecular mutagenesis · 2017Article
- Estrogen Receptor β Agonists Differentially Affect the Growth of Human Melanoma Cell Lines.PloS one · 2015Article
- The comet assay: past, present, and future.Frontiers in genetics · 2015Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The comet assay is a simple and cost effective technique, commonly used to analyze and quantify DNA damage in individual cells. The versatility of the comet assay allows introduction of various modifications to the basic technique. The difference in the methylation sensitivity of the isoschizomeric restriction enzymes HpaII and MspI are used to demonstrate the ability of the comet assay to measure the global DNA methylation level of individual cells when using cell cultures. In the experiments described here, a medium-throughput comet assay and methylation sensitive comet assay are combined to produce a methylation sensitive medium-throughput comet assay to measure changes in the global DNA methylation pattern in individual cells under various growth conditions.
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