ArticleFrontiers in plant science2014
Revealing changes in molecular composition of plant cell walls on the micron-level by Raman mapping and vertex component analysis (VCA).
Article in Frontiers in plant science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 72 citations in OpenAlex.
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- Raman Method in Identification of Species and Varieties, Assessment of Plant Maturity and Crop Quality-A Review.Molecules (Basel, Switzerland) · 2022Review
- Raman spectroscopy mapping of changes in the organization and relative quantities of cell wall polymers in bast fiber cell walls of flax plants exposed to gravitropic stress.Frontiers in plant science · 2022Article
- Correlated mechanochemical maps ofQuantitative plant biology · 2022Article
- Defensive strategies of Norway spruce and Kurile larch heartwood elucidated on the micron-level.Scientific reports · 2021Article
- In Situ Water Quantification in Natural Deep Eutectic Solvents Using Portable Raman Spectroscopy.Molecules (Basel, Switzerland) · 2021Article
- Overview of Popular Techniques of Raman Spectroscopy and Their Potential in the Study of Plant Tissues.Molecules (Basel, Switzerland) · 2021Review
- Laccases and Peroxidases Co-Localize in Lignified Secondary Cell Walls throughout Stem Development.Plant physiology · 2020Article
- Lignans in Knotwood of Norway Spruce: Localisation with Soft X-ray Microscopy and Scanning Transmission Electron Microscopy with Energy Dispersive X-ray Spectroscopy.Molecules (Basel, Switzerland) · 2020Article
- Even Visually Intact Cell Walls in Waterlogged Archaeological Wood Are Chemically Deteriorated and Mechanically Fragile: A Case of a 170 Year-Old Shipwreck.Molecules (Basel, Switzerland) · 2020Article
- Spatial and Temporal Variability of Plant Leaf Responses Cascade after PSII Inhibition: Raman, Chlorophyll Fluorescence and Infrared Thermal Imaging.Sensors (Basel, Switzerland) · 2020Article
- Principles and Applications of Vibrational Spectroscopic Imaging in Plant Science: A Review.Frontiers in plant science · 2020Review
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- Analysis of Cellulose and Lignocellulose Materials by Raman Spectroscopy: A Review of the Current Status.Molecules (Basel, Switzerland) · 2019Review
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- Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus.Plants (Basel, Switzerland) · 2018Article
- Synchrotron Time-Lapse Imaging of Lignocellulosic Biomass Hydrolysis: Tracking Enzyme Localization by Protein Autofluorescence and Biochemical Modification of Cell Walls by Microfluidic Infrared Microspectroscopy.Frontiers in plant science · 2018Article
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1 author at 1 institution in 2 countries.
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Abstract
At the molecular level the plant cell walls consist of a few nanometer thick semi-crystalline cellulose fibrils embedded in amorphous matrix polymers such as pectins, hemicelluloses, and lignins. The arrangement of these molecules within the cell wall in different plant tissues, cells and cell wall layers is of crucial importance for a better understanding and thus optimized utilization of plant biomass. During the last years Confocal Raman microscopy evolved as a powerful method in plant science by revealing the different molecules in context with the microstructure. In this study two-dimensional spectral maps have been acquired of micro-cross-sections of spruce (softwood) and beech (hardwood). Raman images have been derived by using univariate (band integration, height ratios) and multivariate methods [vertex component analysis (VCA)]. While univariate analysis only visualizes changes in selected band heights or areas, VCA separates anatomical regions and cell wall layers with the most different molecular structures. Beside visualization of the distinguished regions and features the underlying molecular structure can be derived based on the endmember spectra. VCA revealed that the lumen sided S3 layer has a similar molecular composition as the pit membrane, both revealing a clear change in lignin composition compared to all other cell wall regions. Within the S2 layer a lamellar structure was visualized, which was elucidated to derive from slight changes in lignin composition and content and might be due to successive but not uniform lignification during growth.
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