Evidence map›Paper›PMID 25071790›Full record

ArticleFrontiers in plant science2014

Photosynthesis in a different light: spectro-microscopy for in vivo characterization of chloroplasts.

Sébastien Peter, Martina B Zell, Christian Blum, Alexander Stuhl, Kirstin Elgass, Marcus Sackrow, Vinod Subramaniam, Alfred J Meixner, Klaus Harter, Veronica G Maurino and 1 more

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In one paragraph

Article in Frontiers in plant science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Sébastien PeterDepartment of Plant Physiology, Center for Plant Molecular Biology (ZMBP), University of Tübingen Tübingen, Germany.
Martina B ZellBiocenter Cologne, Botanical Institute, University of Cologne Cologne, Germany.
Christian BlumNanobiophysics Group and MESA+ Institute for Nanotechnology, University of Twente Enschede, Netherlands.
Alexander StuhlDepartment of Nano Optics, Insitute of Physical and Theoretical Chemistry, University of Tübingen Tübingen, Germany.
Kirstin ElgassDepartment of Plant Physiology, Center for Plant Molecular Biology (ZMBP), University of Tübingen Tübingen, Germany.
Marcus SackrowDepartment of Nano Optics, Insitute of Physical and Theoretical Chemistry, University of Tübingen Tübingen, Germany.
Vinod SubramaniamNanobiophysics Group and MESA+ Institute for Nanotechnology, University of Twente Enschede, Netherlands ; Department of Nanoscale Biophysics, FOM Institute AMOLF Amsterdam, Netherlands.
Alfred J MeixnerDepartment of Nano Optics, Insitute of Physical and Theoretical Chemistry, University of Tübingen Tübingen, Germany.
Klaus HarterDepartment of Plant Physiology, Center for Plant Molecular Biology (ZMBP), University of Tübingen Tübingen, Germany.
Veronica G MaurinoBiocenter Cologne, Botanical Institute, University of Cologne Cologne, Germany ; Plant Molecular Physiology and Biotechnology Group, Institut of Developmental and Molecular Biology of Plants, Cluster of Excellence on Plant Sciences, Heinrich-Heine-Universität Düsseldorf, Germany.
Frank E SchleifenbaumDepartment of Plant Physiology, Center for Plant Molecular Biology (ZMBP), University of Tübingen Tübingen, Germany ; Department of Nano Optics, Insitute of Physical and Theoretical Chemistry, University of Tübingen Tübingen, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During photosynthesis, energy conversion at the two photosystems is controlled by highly complex and dynamic adaptation processes triggered by external factors such as light quality, intensity, and duration, or internal cues such as carbon availability. These dynamics have remained largely concealed so far, because current analytical techniques are based on the investigation of isolated chloroplasts lacking full adaptation ability and are performed at non-physiologically low temperatures. Here, we use non-invasive in planta spectro-microscopic approaches to investigate living chloroplasts in their native environment at ambient temperatures. This is a valuable approach to study the complex function of these systems, because an intrinsic property-the fluorescence emission-is exploited and no additional external perturbations are introduced. Our analysis demonstrates a dynamic adjustment of not only the photosystemI/photosystemII (PSI/PSII) intensity ratio in the chloroplasts but also of the capacity of the LHCs for energy transfer in response to environmental and internal cues.

Indexed as

chloroplastfluorescencephotochemistryphotosystemspectromicroscopyspectroscopy

Identifiers

PMID25071790
PMCPMC4082301

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Registered trials

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