ArticleProteome science2014
Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.
Article in Proteome science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
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- Extracellular matrix protein composition dynamically changes during murine forelimb development.iScience · 2024Article
- How Can Proteomics Help to Elucidate the Pathophysiological Crosstalk in Muscular Dystrophy and Associated Multi-System Dysfunction?Proteomes · 2024Article
- Mass Spectrometry-Based Proteomic Technology and Its Application to Study Skeletal Muscle Cell Biology.Cells · 2023Review
- Identification of Subproteomic Markers for Skeletal Muscle Profiling.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The proteomic profile of the human myotendinous junction.iScience · 2022Article
- mRNA distribution in skeletal muscle is associated with mRNA size.Journal of cell science · 2021Article
- Extracellular matrix at the muscle - tendon interface: functional roles, techniques to explore and implications for regenerative medicine.Connective tissue research · 2021Review
- Single-nucleus transcriptomics reveals functional compartmentalization in syncytial skeletal muscle cells.Nature communications · 2020Article
- Comparative Analysis of the Extracellular Matrix Proteome across the Myotendinous Junction.Journal of proteome research · 2020Article
- Tendon and ligament mechanical loading in the pathogenesis of inflammatory arthritis.Nature reviews. Rheumatology · 2020Review
- Development of myotendinous-like junctions that anchor cardiac valves requires fibromodulin and lumican.Developmental dynamics : an official publication of the American Association of Anatomists · 2016Article
- Anatomical heterogeneity of tendon: Fascicular and interfascicular tendon compartments have distinct proteomic composition.Scientific reports · 2016Article
- Ontogeny informs regeneration: explant models to investigate the role of the extracellular matrix in cartilage tissue assembly and development.Connective tissue researchReview
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The myotendinous junction is a specialized structure of the muscle fibre enriched in mechanosensing complexes, including costameric proteins and core elements of the z-disc. Here, laser capture microdissection was applied to purify membrane regions from the myotendinous junctions of mouse skeletal muscles, which were then processed for proteomic analysis. Sarcolemma sections from the longitudinal axis of the muscle fibre were used as control for the specificity of the junctional preparation. Gene ontology term analysis of the combined lists indicated a statistically significant enrichment in membrane-associated proteins. The myotendinous junction preparation contained previously uncharacterized proteins, a number of z-disc costameric ligands (e.g., actinins, capZ, αB cristallin, filamin C, cypher, calsarcin, desmin, FHL1, telethonin, nebulin, titin and an enigma-like protein) and other proposed players of sarcomeric stretch sensing and signalling, such as myotilin and the three myomesin homologs. A subset were confirmed by immunofluorescence analysis as enriched at the myotendinous junction, suggesting that laser capture microdissection from muscle sections is a valid approach to identify novel myotendinous junction players potentially involved in mechanotransduction pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.