ArticleProceedings of the National Academy of Sciences of the United States of America2014
Spatiotemporal control of epithelial remodeling by regulated myosin phosphorylation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
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Who cites it
75 citing papers in PubMed, 135 citations in OpenAlex.
- Mechanical control in dental and jaw morphogenesis and remodeling.International journal of oral science · 2026Review
- Opposing Activity at the Apical Surface: An Antagonistic Collaboration Between Crumbs and Myosin II Determines Organ Shape.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Article
- Cell rearrangement progression along the apical-basal axis is linked with 3D epithelial tissue structure.PRX life · 2026Article
- Rectification of planar orientation angle switches behavior and replenishes contractile junctions.The Journal of cell biology · 2025Article
- FAS(APO), DAMP, and AKT Phosphoproteins Expression Predict the Development of Nosocomial Infection After Pediatric Burn Injury.Journal of burn care & research : official publication of the American Burn Association · 2024Article
- E-cadherin tunes tissue mechanical behavior before and during morphogenetic tissue flows.Current biology : CB · 2024Article
- Lattice light sheet microscopy reveals 4D force propagation dynamics and leading-edge behaviors in an embryonic epithelium in Drosophila.Current biology : CB · 2024Article
- Conserved physical mechanisms of cell and tissue elongation.Development (Cambridge, England) · 2024Review
- Elastic fibers define embryonic tissue stiffness to enable buckling morphogenesis of the small intestine.Biomaterials · 2023Article
- A chemo-mechanical model of endoderm movements driving elongation of the amniote hindgut.Development (Cambridge, England) · 2023Article
- Serotonin signaling regulates actomyosin contractility during morphogenesis in evolutionarily divergent lineages.Nature communications · 2023Article
- Striped Expression of Leucine-Rich Repeat Proteins Coordinates Cell Intercalation and Compartment Boundary Formation in the EarlySymmetry · 2023Article
- ELASTIC FIBERS DEFINE EMBRYONIC TISSUE STIFFNESS TO ENABLE BUCKLING MORPHOGENESIS OF THE SMALL INTESTINE.bioRxiv : the preprint server for biology · 2023Article
- A chemo-mechanical model of endoderm movements driving elongation of the amniote hindgut.bioRxiv : the preprint server for biology · 2023Article
- aPKC regulates apical constriction to prevent tissue rupture in the Drosophila follicular epithelium.Current biology : CB · 2022Article
- Using optogenetics to link myosin patterns to contractile cell behaviors during convergent extension.Biophysical journal · 2021Article
- Scribble mutation disrupts convergent extension and apical constriction during mammalian neural tube closure.Developmental biology · 2021Article
- Myosin light chain kinase-driven myosin II turnover regulates actin cortex contractility during mitosis.Molecular biology of the cell · 2021Article
- Hexagonal patterning of the Drosophila eye.Developmental biology · 2021Review
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Spatiotemporally regulated actomyosin contractility generates the forces that drive epithelial cell rearrangements and tissue remodeling. Phosphorylation of the myosin II regulatory light chain (RLC) promotes the assembly of myosin monomers into active contractile filaments and is an essential mechanism regulating the level of myosin activity. However, the effects of phosphorylation on myosin localization, dynamics, and function during epithelial remodeling are not well understood. In Drosophila, planar polarized myosin contractility is required for oriented cell rearrangements during elongation of the body axis. We show that regulated myosin phosphorylation influences spatial and temporal properties of contractile behavior at molecular, cellular, and tissue length scales. Expression of myosin RLC variants that prevent or mimic phosphorylation both disrupt axis elongation, but have distinct effects at the molecular and cellular levels. Unphosphorylatable RLC produces fewer, slower cell rearrangements, whereas phosphomimetic RLC accelerates rearrangement and promotes higher-order cell interactions. Quantitative live imaging and biophysical approaches reveal that both phosphovariants reduce myosin planar polarity and mechanical anisotropy, altering the orientation of cell rearrangements during axis elongation. Moreover, the localized myosin activator Rho-kinase is required for spatially regulated myosin activity, even when the requirement for phosphorylation is bypassed by the expression of phosphomimetic myosin RLC. These results indicate that myosin phosphorylation influences both the level and the spatiotemporal regulation of myosin activity, linking molecular properties of myosin activity to tissue morphogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.