ArticleProceedings of the National Academy of Sciences of the United States of America2014
Development of an antibody-based, modular biosensor for 129Xe NMR molecular imaging of cells at nanomolar concentrations.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 110 citations in OpenAlex.
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- Hyperpolarized Xenon-129 Chemical Exchange Saturation Transfer (HyperCEST) Molecular Imaging: Achievements and Future Challenges.International journal of molecular sciences · 2024Review
- Spin Hyperpolarization in Modern Magnetic Resonance.Chemical reviews · 2023Review
- Hyper-CEST NMR of metal organic polyhedral cages reveals hidden diastereomers with diverse guest exchange kinetics.Nature communications · 2022Article
- Ultrasensitive molecular building block for biothiol NMR detection at picomolar concentrations.iScience · 2021Article
- Versatile non-luminescent color palette based on guest exchange dynamics in paramagnetic cavitands.Nature communications · 2021Article
- Identification of extracellular nanoparticle subsets by nuclear magnetic resonance.Chemical science · 2021Article
- Mapping of Absolute Host Concentration and Exchange Kinetics of Xenon Hyper-CEST MRI Agents.Pharmaceuticals (Basel, Switzerland) · 2021Article
- Cryptophane-xenon complexes forRSC advances · 2021Article
- Article
- Molecular Sensing with Host Systems for HyperpolarizedMolecules (Basel, Switzerland) · 2020Review
- Paramagnetic Organocobalt Capsule Revealing Xenon Host-Guest Chemistry.Inorganic chemistry · 2020Article
- Detecting protein-protein interactions by Xe-129 NMR.Chemical communications (Cambridge, England) · 2020Article
- Paramagnetic Shifts and Guest Exchange Kinetics in CoInorganic chemistry · 2020Article
- Hyperpolarized Xe NMR signal advancement by metal-organic framework entrapment in aqueous solution.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Opportunities and Challenges for Biosensors and Nanoscale Analytical Tools for Pandemics: COVID-19.ACS nano · 2020Review
- Probing Reversible Guest Binding with HyperpolarizedMolecules (Basel, Switzerland) · 2020Review
- Directly Functionalized Cucurbit[7]uril as a Biosensor for the Selective Detection of Protein Interactions byChemistry (Weinheim an der Bergstrasse, Germany) · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Magnetic resonance imaging (MRI) is seriously limited when aiming for visualization of targeted contrast agents. Images are reconstructed from the weak diamagnetic properties of the sample and require an abundant molecule like water as the reporter. Micromolar to millimolar concentrations of conventional contrast agents are needed to generate image contrast, thus excluding many molecular markers as potential targets. To address this limitation, we developed and characterized a functional xenon NMR biosensor that can identify a specific cell surface marker by targeted (129)Xe MRI. Cells expressing the cell surface protein CD14 can be spatially distinguished from control cells with incorporation of as little as 20 nM of the xenon MRI readout unit, cryptophane-A. Cryptophane-A serves as a chemical host for hyperpolarized nuclei and facilitates the sensitivity enhancement achieved by xenon MRI. Although this paper describes the application of a CD14-specific biosensor, the construct has been designed in a versatile, modular fashion. This allows for quick and easy adaptation of the biosensor to any cell surface target for which there is a specific antibody. In addition, the modular design facilitates the creation of a multifunctional probe that incorporates readout modules for different detection methods, such as fluorescence, to complement the primary MRI readout. This modular antibody-based approach not only offers a practical technique with which to screen targets, but one which can be readily applied as the xenon MRI field moves closer to molecular imaging applications in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.