SynthesisProceedings of the National Academy of Sciences of the United States of America2014
Calorie restriction does not elicit a robust extension of replicative lifespan in Saccharomyces cerevisiae.
Synthesis in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 51 citations in OpenAlex.
- Article
- Calorie Restriction Decreases Competitive Fitness inMicroorganisms · 2024Article
- Molecular Mechanisms of Genotype-Dependent Lifespan Variation Mediated by Caloric Restriction: Insight from Wild Yeast Isolates.bioRxiv : the preprint server for biology · 2024Article
- Molecular mechanisms of genotype-dependent lifespan variation mediated by caloric restriction: insight from wild yeast isolates.Frontiers in aging · 2024Article
- Measuring Replicative Lifespan in Cryptococcus neoformans.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Dietary change without caloric restriction maintains a youthful profile in ageing yeast.PLoS biology · 2023Article
- Quantification methods of determining brewer's and pharmaceutical yeast cell viability: accuracy and impact of nanoparticles.Analytical and bioanalytical chemistry · 2023Article
- Fungi-on-a-Chip: microfluidic platforms for single-cell studies on fungi.FEMS microbiology reviews · 2022Review
- DetecDiv, a generalist deep-learning platform for automated cell division tracking and survival analysis.eLife · 2022Article
- rDNA array length is a major determinant of replicative lifespan in budding yeast.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Modeling aging and its impact on cellular function and organismal behavior.Experimental gerontology · 2021Review
- Potassium and Sodium Salt Stress Characterization in the Yeasts Saccharomyces cerevisiae, Kluyveromyces marxianus, andApplied and environmental microbiology · 2021Article
- Slipstreaming Mother Machine: A Microfluidic Device for Single-Cell Dynamic Imaging of Yeast.Micromachines · 2020Article
- Life span extension by glucose restriction is abrogated by methionine supplementation: Cross-talk between glucose and methionine and implication of methionine as a key regulator of life span.Science advances · 2020Article
- Article
- An inexpensive microscopy system for microfluidic studies in budding yeast.Translational medicine of aging · 2019Article
- The role of autophagy in the regulation of yeast life span.Annals of the New York Academy of Sciences · 2018Review
- Putting microfluidics in other people's hands.Nature methods · 2018Article
- Behead and live long or the tale of cathepsin L.Yeast (Chichester, England) · 2018Article
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calorie restriction (CR) is often described as the most robust manner to extend lifespan in a large variety of organisms. Hence, considerable research effort is directed toward understanding the mechanisms underlying CR, especially in the yeast Saccharomyces cerevisiae. However, the effect of CR on lifespan has never been systematically reviewed in this organism. Here, we performed a meta-analysis of replicative lifespan (RLS) data published in more than 40 different papers. Our analysis revealed that there is significant variation in the reported RLS data, which appears to be mainly due to the low number of cells analyzed per experiment. Furthermore, we found that the RLS measured at 2% (wt/vol) glucose in CR experiments is partly biased toward shorter lifespans compared with identical lifespan measurements from other studies. Excluding the 2% (wt/vol) glucose experiments from CR experiments, we determined that the average RLS of the yeast strains BY4741 and BY4742 is 25.9 buds at 2% (wt/vol) glucose and 30.2 buds under CR conditions. RLS measurements with a microfluidic dissection platform produced identical RLS data at 2% (wt/vol) glucose. However, CR conditions did not induce lifespan extension. As we excluded obvious methodological differences, such as temperature and medium, as causes, we conclude that subtle method-specific factors are crucial to induce lifespan extension under CR conditions in S. cerevisiae.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.