ArticleThe Journal of biological chemistry2014
Protein kinase Cα (PKCα) regulates bone architecture and osteoblast activity.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Tensile stress promotes the chondrogenic ability of condylar cartilage stem/progenitor cells in the temporomandibular joint via the Piezo1-CaStem cell research & therapy · 2025Article
- The Role of Protein Kinase C During the Differentiation of Stem and Precursor Cells into Tissue Cells.Biomedicines · 2024Review
- Differential gene expression in the calvarial and cortical bone of juvenile female mice.Frontiers in endocrinology · 2023Article
- Distinct role of mitochondrial function and protein kinase C in intimal and medial calcificationFrontiers in cardiovascular medicine · 2022Article
- Phorbol-12-myristate 13-acetate inhibits Nephronectin gene expression via Protein kinase C alpha and c-Jun/c-Fos transcription factors.Scientific reports · 2021Article
- Classical isoforms of protein kinase C (PKC) and Akt regulate the osteogenic differentiation of human dental follicle cells via both β-catenin and NF-κB.Stem cell research & therapy · 2021Article
- Mechanical strain-mediated reduction in RANKL expression is associated with RUNX2 and BRD2.Gene: X · 2020Article
- A novel computational strategy for DNA methylation imputation using mixture regression model (MRM).BMC bioinformatics · 2020Article
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- Regulation of vascular smooth muscle cell calcification by syndecan-4/FGF-2/PKCα signalling and cross-talk with TGFβ.Cardiovascular research · 2017Article
- Regulatory Variants Modulate Protein Kinase C α (PRKCA) Gene Expression in Human Heart.Pharmaceutical research · 2017Article
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- Genome-wide association of multiple complex traits in outbred mice by ultra-low-coverage sequencing.Nature genetics · 2016Article
- The Essentiality of Arachidonic Acid in Infant Development.Nutrients · 2016Review
- Wnt16 Is Associated with Age-Related Bone Loss and Estrogen Withdrawal in Murine Bone.PloS one · 2015Article
- Quantification of Alterations in Cortical Bone Geometry Using Site Specificity Software in Mouse models of Aging and the Responses to Ovariectomy and Altered Loading.Frontiers in endocrinology · 2015Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Bones' strength is achieved and maintained through adaptation to load bearing. The role of the protein kinase PKCα in this process has not been previously reported. However, we observed a phenotype in the long bones of Prkca(-/-) female but not male mice, in which bone tissue progressively invades the medullary cavity in the mid-diaphysis. This bone deposition progresses with age and is prevented by disuse but unaffected by ovariectomy. Castration of male Prkca(-/-) but not WT mice results in the formation of small amounts of intramedullary bone. Osteoblast differentiation markers and Wnt target gene expression were up-regulated in osteoblast-like cells derived from cortical bone of female Prkca(-/-) mice compared with WT. Additionally, although osteoblastic cells derived from WT proliferate following exposure to estradiol or mechanical strain, those from Prkca(-/-) mice do not. Female Prkca(-/-) mice develop splenomegaly and reduced marrow GBA1 expression reminiscent of Gaucher disease, in which PKC involvement has been suggested previously. From these data, we infer that in female mice, PKCα normally serves to prevent endosteal bone formation stimulated by load bearing. This phenotype appears to be suppressed by testicular hormones in male Prkca(-/-) mice. Within osteoblastic cells, PKCα enhances proliferation and suppresses differentiation, and this regulation involves the Wnt pathway. These findings implicate PKCα as a target gene for therapeutic approaches in low bone mass conditions.
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