ArticleMethods in molecular biology (Clifton, N.J.)2014
Optogenetic control of cardiomyocytes via viral delivery.
Article in Methods in molecular biology (Clifton, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 59 citations in OpenAlex.
- Response of human iPSC-cardiomyocytes to adrenergic drugs assessed by high-throughput pericellular oxygen measurements and computational modeling.Journal of molecular and cellular cardiology plus · 2026Article
- Multimodal Layer-Crossing Interrogation of Brain Circuits Enabled by Microfluidic Axialtrodes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Cardiac optogenetics: shining light on signaling pathways.Pflugers Archiv : European journal of physiology · 2023Review
- Opto-SICM framework combines optogenetics with scanning ion conductance microscopy for probing cell-to-cell contacts.Communications biology · 2023Article
- Portable low-cost macroscopic mapping system for all-optical cardiac electrophysiology.Journal of biomedical optics · 2023Article
- Protein and mRNA Quantification in Small Samples of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes in 96-Well Microplates.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Cardiac optogenetics: a decade of enlightenment.Nature reviews. Cardiology · 2021Review
- OptoGap is an optogenetics-enabled assay for quantification of cell-cell coupling in multicellular cardiac tissue.Scientific reports · 2021Article
- Astrocytes in the Ventromedial Hypothalamus Involve Chronic Stress-Induced Anxiety and Bone Loss in Mice.Neural plasticity · 2021Article
- Integration of Engineered "Spark-Cell" Spheroids for Optical Pacing of Cardiac Tissue.Frontiers in bioengineering and biotechnology · 2021Article
- Microfluidics-enabled 96-well perfusion system for high-throughput tissue engineering and long-term all-optical electrophysiology.Lab on a chip · 2020Article
- Syncytium cell growth increases Kir2.1 contribution in human iPSC-cardiomyocytes.American journal of physiology. Heart and circulatory physiology · 2020Article
- Feasibility of Using Adjunctive Optogenetic Technologies in Cardiomyocyte Phenotyping - from the Single Cell to the Whole Heart.Current pharmaceutical biotechnology · 2020Review
- A Software Architecture to Mimic a Ventricular Tachycardia in Intact Murine Hearts by Means of an All-Optical Platform.Methods and protocols · 2019Article
- Adeno-Associated Virus Mediated Gene Delivery: Implications for ScalableFrontiers in physiology · 2019Article
- Light-Activated Dynamic Clamp Using iPSC-Derived Cardiomyocytes.Biophysical journal · 2018Article
- Real-time optical manipulation of cardiac conduction in intact hearts.The Journal of physiology · 2018Article
- Induction of retinal-dependent calcium influx in human melanocytes by UVA or UVB radiation contributes to the stimulation of melanosome transfer.Cell proliferation · 2017Article
- Evaluation of Optogenetic Electrophysiology Tools in Human Stem Cell-Derived Cardiomyocytes.Frontiers in physiology · 2017Article
- OptoDyCE as an automated system for high-throughput all-optical dynamic cardiac electrophysiology.Nature communications · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Optogenetics is an emerging technology for the manipulation and control of excitable tissues, such as the brain and heart. As this technique requires the genetic modification of cells in order to inscribe light sensitivity, for cardiac applications, here we describe the process through which neonatal rat ventricular myocytes are virally infected in vitro with channelrhodopsin-2 (ChR2). We also describe in detail the procedure for quantitatively determining the optimal viral dosage, including instructions for patterning gene expression in multicellular cardiomyocyte preparations (cardiac syncytia) to simulate potential in vivo transgene distributions. Finally, we address optical actuation of ChR2-transduced cells and means to measure their functional response to light.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.