Evidence map›Paper›PMID 25070008›Full record

ArticleDiagnostic microbiology and infectious disease2014

Comparison of a new transport medium with universal transport medium at a tropical field site.

Elizabeth P Schlaudecker, Joan P Heck, Elizabeth T MacIntyre, Ruben Martinez, Caitlin N Dodd, Monica M McNeal, Mary A Staat, Jeffery E Heck, Mark C Steinhoff

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Diagnostic microbiology and infectious disease, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Guideline
  2. Trial
  3. Trial
  4. Article
  5. Modified transport medium for improving influenza virus detection.Frontiers in cellular and infection microbiology · 2024
    Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Elizabeth P SchlaudeckerDivision of Infectious Diseases, Global Health Center, Cincinnati Children's Hospital and Medical Center, Cincinnati, OH, USA. Electronic address: Elizabeth.Schlaudecker@cchmc.org.
Joan P HeckShoulder to Shoulder, Santa Lucia, Intibucá, Honduras.
Elizabeth T MacIntyreShoulder to Shoulder, Santa Lucia, Intibucá, Honduras.
Ruben MartinezShoulder to Shoulder, Santa Lucia, Intibucá, Honduras.
Caitlin N DoddGlobal Health Center, Cincinnati Children's Hospital and Medical Center, Cincinnati, OH, USA.
Monica M McNealDivision of Infectious Diseases, Cincinnati Children's Hospital and Medical Center, Cincinnati, OH, USA.
Mary A StaatDivision of Infectious Diseases, Cincinnati Children's Hospital and Medical Center, Cincinnati, OH, USA.
Jeffery E HeckDepartment of Family Medicine, University of North Carolina, Asheville, NC, USA.
Mark C SteinhoffDivision of Infectious Diseases, Global Health Center, Cincinnati Children's Hospital and Medical Center, Cincinnati, OH, USA.

Funding

Fogarty International Clinical Research Scholars Support Center @ Vanderbilt-AAMCR24TW007988 · FIC · VANDERBILT UNIVERSITY · PI VERMUND, STEN H. · 2007 to 2011
$27.6M
CTSA INFRASTRUCTURE FOR PEDIATRIC RESEARCHUL1RR026314 · NCRR · UNIVERSITY OF CINCINNATI · PI HEUBI, JAMES E. · 2009 to 2011
$14.3M
Pediatric Center--Gene Expression /Developmental ScienceK12HD028827 · NICHD · CINCINNATI CHILDRENS HOSP MED CTR · PI Tina Lee Cheng · 2002 to 2026
$9.6M
FIC NIH HHS 5 R24 TW007988-03FIC NIH HHS R24 TW007988NCRR NIH HHS 5UL1RR026314-3NCRR NIH HHS UL1 RR026314NICHD NIH HHS K12 HD028827
6 · The paper itself

Abstract

Limited data are available in rural Honduran settings describing the etiology of respiratory infections, partially due to limited specimen transport. A new molecular transport media (MTM) preserves released nucleic acid at ambient temperature for later detection. Prospective surveillance was conducted in a Honduran clinic to identify 233 children less than 5 years of age presenting with respiratory symptoms. We obtained 2 nasopharyngeal samples and stored 1 in PrimeStore® MTM at room temperature and 1 in universal transport media (UTM) at -80 °C. The specimens were then transported to Cincinnati Children's Hospital and tested for 16 respiratory viruses using a multiplex PCR panel. The 2 specimen collection systems were similar for detecting the 4 most common viruses: influenza (Kappa = 0.7676, P < 0.0001), human metapneumovirus (Kappa = 0.8770, P < 0.0001), respiratory syncytial virus (Kappa = 0.6849, P < 0.0001), and parainfluenza (Kappa = 0.8796, P < 0.0001). These results suggest that clinical specimens transported via PrimeStore® MTM and UTM yield similar viral multiplex PCR results.

Indexed as

Child, PreschoolCulture MediaFemaleHondurasHumansInfantInfant, NewbornMaleMolecular Diagnostic TechniquesMultiplex Polymerase Chain ReactionProspective StudiesRespiratory Tract InfectionsSpecimen HandlingTemperatureVirus DiseasesVirusesCulture MediaInfluenzaTransport medium

Identifiers

PMID25070008
PMCPMC4164576

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.