ArticleMolecular microbiology2014
Identification of host genes that affect acquisition of an integrative and conjugative element in Bacillus subtilis.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 67 citations in OpenAlex.
- The phenotypic landscape of the model firmicuteProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Clinical Rel mutations that increase basal (p)ppGpp promote conjugal transfer of staphylococcal resistance plasmids.Microbiology (Reading, England) · 2026Article
- Nucleoside binding by a surface lipoprotein governs conjugative ICE acquisition in mycoplasmas.mBio · 2026Article
- Article
- Cross-species phenotypic profiling uncovers functional determinants of bacterial cold shock adaptation.bioRxiv : the preprint server for biology · 2025Article
- The extended mobility of plasmids.Nucleic acids research · 2025Review
- A shared alarmone-GTP switch controls persister formation in bacteria.Nature microbiology · 2025Article
- A phage-encoded counter-defense inhibits an NAD-degrading anti-phage defense system.PLoS genetics · 2025Article
- Identification of insertion sites for the integrative and conjugative element Tn916 in the Bacillus subtilis chromosome.PloS one · 2025Article
- Transcription termination and antitermination are critical for the fitness and function of the integrative and conjugative element Tn916.PLoS genetics · 2024Article
- YfmR is a translation factor that prevents ribosome stalling and cell death in the absence of EF-P.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- YfmR is a translation factor that prevents ribosome stalling and cell death in the absence of EF-P.bioRxiv : the preprint server for biology · 2023Article
- Recipient UvrD helicase is involved in single- to double-stranded DNA conversion during conjugative plasmid transfer.Nucleic acids research · 2023Article
- TnSmu1 is a functional integrative and conjugative element in Streptococcus mutans that when expressed causes growth arrest of host bacteria.Molecular microbiology · 2022Article
- Beneficial and detrimental genes in the cellular response to replication arrest.PLoS genetics · 2022Article
- Activation of the integrative and conjugative element Tn916 causes growth arrest and death of host bacteria.PLoS genetics · 2022Article
- Intrinsically disordered protein regions are required for cell wall homeostasis inGenes & development · 2022Article
- Identification of Genes Required for Swarming Motility inJournal of bacteriology · 2022Article
- A CRISPR interference screen reveals a role for cell wall teichoic acids in conjugation in Bacillus subtilis.Molecular microbiology · 2022Article
- Biology and engineering of integrative and conjugative elements: Construction and analyses of hybrid ICEs reveal element functions that affect species-specific efficiencies.PLoS genetics · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Conjugation, a major type of horizontal gene transfer in bacteria, involves transfer of DNA from a donor to a recipient using donor-encoded conjugation machinery. Using a high-throughput screen (Tn-seq), we identified genes in recipients that contribute to acquisition of the integrative and conjugative element ICEBs1 by Bacillus subtilis. We found that null mutations in some genes caused an increase, and others a decrease in conjugation efficiency. Some mutations affected conjugation only when present in recipients. Other mutations affected conjugation when present in donors or recipients. Most of the genes identified are known or predicted to affect the cell envelope. Several encode enzymes involved in phospholipid biosynthesis and one encodes a homologue of penicillin-binding proteins. Two of the genes identified also affected conjugation of Tn916, indicating that their roles in conjugation may be general. We did not identify any genes in recipients that were essential for ICEBs1 conjugation, indicating that if there are such genes, then these are either essential for cell growth or redundant. Our results indicate that acquisition of ICEBs1, and perhaps other conjugative elements, is robust and not easily avoided by mutation and that several membrane-related functions affect the efficiency of conjugation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.