ArticleScientific reports2014
Identification and use of an alkane transporter plug-in for applications in biocatalysis and whole-cell biosensing of alkanes.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed.
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- Adaptive Laboratory Evolution Unlocks Membrane Permeability as a Key Limitation in Long-Chain Alcohol Metabolism by Pseudomonas putida KT2440.Microbial biotechnology · 2026Article
- Quantitative Proteomic Analysis of Differential Proteins from Acinetobacter sp. HC8-3S Responses to Hydrocarbons.Current microbiology · 2026Article
- Evolution of Methods for the Oxidation of Primary Alcohols to Carboxylic Acids: From Metal Oxides to Biocatalysis.JACS Au · 2026Review
- Directed evolution of hydrocarbon-producing enzymes.Biotechnology for biofuels and bioproducts · 2025Review
- Quantifying bacterial efflux within subcellular domains ofApplied and environmental microbiology · 2024Article
- Development of a whole-cell biosensor for ethylene oxide and ethylene.Microbial biotechnology · 2024Article
- Strategies found not to be suitable for stabilizing high steroid hydroxylation activities of CYP450 BM3-based whole-cell biocatalysts.PloS one · 2024Article
- Structure and Function of Alkane Monooxygenase (AlkB).Accounts of chemical research · 2023Review
- A Novel FadL Homolog, AltL, Mediates Transport of Long-Chain Alkanes and Fatty Acids in Acinetobacter venetianus RAG-1.Applied and environmental microbiology · 2022Article
- Efficient Synthesis of (Frontiers in bioengineering and biotechnology · 2022Article
- Investigation of the prevalence and catalytic activity of rubredoxin-fused alkane monooxygenases (AlkBs).Journal of inorganic biochemistry · 2021Article
- Synthetic Biology Approaches to Hydrocarbon Biosensors: A Review.Frontiers in bioengineering and biotechnology · 2021Review
- Comparative Proteomics ofMicroorganisms · 2020Article
- A β-barrel for oil transport through lipid membranes: Dynamic NMR structures of AlkL.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Production of 1-Dodecanol, 1-Tetradecanol, and 1,12-Dodecanediol through Whole-Cell Biotransformation in Escherichia coli.Applied and environmental microbiology · 2018Article
- Differential Protein Expression During Growth on Medium Versus Long-Chain Alkanes in the Obligate Marine Hydrocarbon-Degrading BacteriumFrontiers in microbiology · 2018Article
- Effect of cell permeability and dehydrogenase expression on octane activation by CYP153A6-based whole cell Escherichia coli catalysts.Microbial cell factories · 2017Article
- Whole-cell biocatalysts by design.Microbial cell factories · 2017Review
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Authors and funding
11 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Effective application of whole-cell devices in synthetic biology and biocatalysis will always require consideration of the uptake of molecules of interest into the cell. Here we demonstrate that the AlkL protein from Pseudomonas putida GPo1 is an alkane import protein capable of industrially relevant rates of uptake of C7-C16 n-alkanes. Without alkL expression, native E.coli n-alkane uptake was the rate-limiting step in both the whole-cell bioconversion of C7-C16 n-alkanes and in the activation of a whole-cell alkane biosensor by C10 and C11 alkanes. By coexpression of alkL as a transporter plug-in, specific yields improved by up to 100-fold for bioxidation of >C12 alkanes to fatty alcohols and acids. The alkL protein was shown to be toxic to the host when overexpressed but when expressed from a vector capable of controlled induction, yields of alkane oxidation were improved a further 10-fold (8 g/L and 1.7 g/g of total oxidized products). Further testing of activity on n-octane with the controlled expression vector revealed the highest reported rates of 120 μmol/min/g and 1 g/L/h total oxidized products. This is the first time AlkL has been shown to directly facilitate enhanced uptake of C10-C16 alkanes and represents the highest reported gain in product yields resulting from its use.
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