Evidence map›Paper›PMID 25068100›Full record

ArticleActa pharmaceutica Sinica. B2012

Cytochrome P450 2C24: Expression, Tissue Distribution, High-Throughput Assay, and Pharmacological Inhibition.

Jun Yang, Melissa A VanAlstine, James G Phillips, Mark P Wentland, Lindsay B Hough

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta pharmaceutica Sinica. B, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 90% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Jun YangCenter for Neuropharmacology and Neuroscience, Albany Medical College, Albany, NY 12208, USA.
Melissa A VanAlstineCenter for Neuropharmacology and Neuroscience, Albany Medical College, Albany, NY 12208, USA.
James G PhillipsCurragh Chemistries, Cleveland, OH, USA.
Mark P WentlandDept. of Chemistry, Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, New York, USA.
Lindsay B HoughCenter for Neuropharmacology and Neuroscience, Albany Medical College, Albany, NY 12208, USA.
Albany Medical Center Hospital · USCleveland Research (United States) · US

Funding

P450 Epoxygenase Mechanisms of Opioid AnalgesiaR01DA027835 · NIDA · ALBANY MEDICAL COLLEGE · PI HOUGH, LINDSAY · 2010 to 2014
$1.6M
NIDA NIH HHS R01 DA027835
6 · The paper itself

Abstract

Cytochrome P450 (CYP)-mediated epoxidation of arachidonic acid (AA) contributes to important biological functions, including the pain-relieving responses produced by analgesic drugs. However, the relevant epoxygenase(s) remain unidentified. Presently, we describe the tissue distribution, high-throughput assay, and pharmacological characteristics of the rat epoxygenase CYP2C24. Following cloning from male rat liver, recombinant baculovirus containing the C-terminal His-tagged cDNA was constructed and used to express the protein in Spodoptera frugiperda (Sf9) cells. Enzymatic activity was detected with membranes, NADPH regenerating system and CYP reductase, and optimized for high throughput screening by use of the Vivid Blue

Indexed as

analgesiabrainCytochrome P450 2C24Cytochrome P450 2C55P450 inhibitorspain

Identifiers

PMID25068100
PMCPMC4108250
OpenAlexW2036172155

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.