ArticleMolecular cell2014
Hierarchical molecular events driven by oocyte-specific factors lead to rapid and extensive reprogramming.
Article in Molecular cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 44 citations in OpenAlex.
- Reversibility, regulation, and the community of development: the legacy of Sir John B. Gurdon.Nature communications · 2025Review
- The Mammalian Oocyte: A Central Hub for Cellular Reprogramming and Stemness.Stem cells and cloning : advances and applications · 2025Review
- The first chicken oocyte nucleus whole transcriptomic profile defines the spectrum of maternal mRNA and non-coding RNA genes transcribed by the lampbrush chromosomes.Nucleic acids research · 2024Article
- Deterministic nuclear reprogramming of mammalian nuclei to a totipotency-like state by Amphibian meiotic oocytes for stem cell therapy in humans.Biology open · 2024Article
- WD repeat domain 82 (Wdr82) facilitates mouse iPSCs generation by interfering mitochondrial oxidative phosphorylation and glycolysis.Cellular and molecular life sciences : CMLS · 2023Article
- The Oocyte-Specific Linker Histone H1FOO Is Not Essential for Mouse Oogenesis and Fertility.Cells · 2022Article
- Cell division- and DNA replication-free reprogramming of somatic nuclei for embryonic transcription.iScience · 2021Article
- Embryonic fate after somatic cell nuclear transfer in non-enucleated goldfish oocytes is determined by first cleavages and DNA methylation patterns.Scientific reports · 2021Article
- The Role of CaFrontiers in cell and developmental biology · 2021Article
- Analysis of Menstrual Blood Stromal Cells Reveals SOX15 Triggers Oocyte-Based Human Cell Reprogramming.iScience · 2020Article
- Long-term association of a transcription factor with its chromatin binding site can stabilize gene expression and cell fate commitment.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The embryonic linker histone dBigH1 alters the functional state of active chromatin.Nucleic acids research · 2020Article
- Histone Lysine Demethylases KDM5B and KDM5C Modulate Genome Activation and Stability in Porcine Embryos.Frontiers in cell and developmental biology · 2020Article
- Various nuclear reprogramming systems using egg and oocyte materials.The Journal of reproduction and development · 2019Review
- Shaping Gene Expression by Landscaping Chromatin Architecture: Lessons from a Master.Molecular cell · 2018Review
- Chromatin remodeling in Drosophila preblastodermic embryo extract.Scientific reports · 2018Article
- Chromatin Accessibility Impacts Transcriptional Reprogramming in Oocytes.Cell reports · 2018Article
- Cancer reversion with oocyte extracts is mediated by cell cycle arrest and induction of tumour dormancy.Oncotarget · 2018Article
- Oocyte-Specific Homeobox 1, Obox1, Facilitates Reprogramming by Promoting Mesenchymal-to-Epithelial Transition and Mitigating Cell Hyperproliferation.Stem cell reports · 2017Article
- Gene Resistance to Transcriptional Reprogramming following Nuclear Transfer Is Directly Mediated by Multiple Chromatin-Repressive Pathways.Molecular cell · 2017Article
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
Abstract
Nuclear transfer to oocytes is an efficient way to transcriptionally reprogram somatic nuclei, but its mechanisms remain unclear. Here, we identify a sequence of molecular events that leads to rapid transcriptional reprogramming of somatic nuclei after transplantation to Xenopus oocytes. RNA-seq analyses reveal that reprogramming by oocytes results in a selective switch in transcription toward an oocyte rather than pluripotent type, without requiring new protein synthesis. Time-course analyses at the single-nucleus level show that transcriptional reprogramming is induced in most transplanted nuclei in a highly hierarchical manner. We demonstrate that an extensive exchange of somatic- for oocyte-specific factors mediates reprogramming and leads to robust oocyte RNA polymerase II binding and phosphorylation on transplanted chromatin. Moreover, genome-wide binding of oocyte-specific linker histone B4 supports its role in transcriptional reprogramming. Thus, our study reveals the rapid, abundant, and stepwise loading of oocyte-specific factors onto somatic chromatin as important determinants for successful reprogramming.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.