ArticleNucleic acids research2014
Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
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- Highly multiplexed oligonucleotide probe-ligation testing enables efficient extraction-free SARS-CoV-2 detection and viral genotyping.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2021Article
- Highly multiplexed oligonucleotide probe-ligation testing enables efficient extraction-free SARS-CoV-2 detection and viral genotyping.bioRxiv : the preprint server for biology · 2020Article
- High-throughput screening and classification of chemicals and their effects on neuronal gene expression using RASL-seq.Scientific reports · 2019Article
- Identification of Androgen Receptor Modulators in a Prostate Cancer Cell Line Microarray Compendium.Toxicological sciences : an official journal of the Society of Toxicology · 2018Article
- Multiplexed analysis of fixed tissue RNA using Ligation in situ Hybridization.Nucleic acids research · 2017Article
- A Tox21 Approach to Altered Epigenetic Landscapes: Assessing Epigenetic Toxicity Pathways Leading to Altered Gene Expression and Oncogenic Transformation In Vitro.International journal of molecular sciences · 2017Review
- Tradict enables accurate prediction of eukaryotic transcriptional states from 100 marker genes.Nature communications · 2017Article
- Archaeal RNA ligase from thermoccocus kodakarensis for template dependent ligation.RNA biology · 2017Article
- A trichostatin A expression signature identified by TempO-Seq targeted whole transcriptome profiling.PloS one · 2017Article
- A chemical-biological similarity-based grouping of complex substances as a prototype approach for evaluating chemical alternatives.Green chemistry : an international journal and green chemistry resource : GC · 2016Article
- Sensitive and specific miRNA detection method using SplintR Ligase.Nucleic acids research · 2016Article
- Moving Toward Integrating Gene Expression Profiling Into High-Throughput Testing: A Gene Expression Biomarker Accurately Predicts Estrogen Receptor α Modulation in a Microarray Compendium.Toxicological sciences : an official journal of the Society of Toxicology · 2016Article
- Article
- Conserved proline-directed phosphorylation regulates SR protein conformation and splicing function.The Biochemical journal · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
A sensitive and highly multiplex method to directly measure RNA sequence abundance without requiring reverse transcription would be of value for a number of biomedical applications, including high throughput small molecule screening, pathogen transcript detection and quantification of short/degraded RNAs. R NA A: nnealing, S: election and L: igation (RASL) assays, which are based on RNA template-dependent oligonucleotide probe ligation, have been developed to meet this need, but technical limitations have impeded their adoption. Whereas DNA ligase-based RASL assays suffer from extremely low and sequence-dependent ligation efficiencies that compromise assay robustness, Rnl2 can join a fully DNA donor probe to a 3'-diribonucleotide-terminated acceptor probe with high efficiency on an RNA template strand. Rnl2-based RASL exhibits sub-femtomolar transcript detection sensitivity, and permits the rational tuning of probe signals for optimal analysis by massively parallel DNA sequencing (RASL-seq). A streamlined Rnl2-based RASL-seq protocol was assessed in a small molecule screen using 77 probe sets designed to monitor complex human B cell phenotypes during antibody class switch recombination. Our data demonstrate the robustness, cost-efficiency and broad applicability of Rnl2-based RASL assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.