ArticleBMC developmental biology2014
Proteomic analysis of fibroblastema formation in regenerating hind limbs of Xenopus laevis froglets and comparison to axolotl.
Article in BMC developmental biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- PLOD2, a key factor for MRL MSC metabolism and chondroprotective properties.Stem cell research & therapy · 2024Article
- The salamander blastema within the broader context of metazoan regeneration.Frontiers in cell and developmental biology · 2023Review
- Acute multidrug delivery via a wearable bioreactor facilitates long-term limb regeneration and functional recovery in adultScience advances · 2022Article
- Evi5 is required forFrontiers in cell and developmental biology · 2022Article
- Article
- Melanocortin Receptor 4 Signaling Regulates Vertebrate Limb Regeneration.Developmental cell · 2018Article
- Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing.Scientific reports · 2018Article
- Mechanisms of urodele limb regeneration.Regeneration (Oxford, England) · 2017Review
- Ion channel signaling influences cellular proliferation and phagocyte activity during axolotl tail regeneration.Mechanisms of development · 2017Article
- Msh homeobox 1 (The Journal of biological chemistry · 2017Article
- Tetrapod limb and sarcopterygian fin regeneration share a core genetic programme.Nature communications · 2016Article
- LimiTT: link miRNAs to targets.BMC bioinformatics · 2016Article
- Frogs as integrative models for understanding digestive organ development and evolution.Seminars in cell & developmental biology · 2016Review
- A Tunable Silk Hydrogel Device for Studying Limb Regeneration in Adult Xenopus Laevis.PloS one · 2016Article
- Budgett's frog (Lepidobatrachus laevis): A new amphibian embryo for developmental biology.Developmental biology · 2015Article
- Drug-induced regeneration in adult mice.Science translational medicine · 2015Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTo gain insight into what differences might restrict the capacity for limb regeneration in Xenopus froglets, we used High Performance Liquid Chromatography (HPLC)/double mass spectrometry to characterize protein expression during fibroblastema formation in the amputated froglet hindlimb, and compared the results to those obtained previously for blastema formation in the axolotl limb.
resultsComparison of the Xenopus fibroblastema and axolotl blastema revealed several similarities and significant differences in proteomic profiles. The most significant similarity was the strong parallel down regulation of muscle proteins and enzymes involved in carbohydrate metabolism. Regenerating Xenopus limbs differed significantly from axolotl regenerating limbs in several ways: deficiency in the inositol phosphate/diacylglycerol signaling pathway, down regulation of Wnt signaling, up regulation of extracellular matrix (ECM) proteins and proteins involved in chondrocyte differentiation, lack of expression of a key cell cycle protein, ecotropic viral integration site 5 (EVI5), that blocks mitosis in the axolotl, and the expression of several patterning proteins not seen in the axolotl that may dorsalize the fibroblastema.
conclusionsWe have characterized global protein expression during fibroblastema formation after amputation of the Xenopus froglet hindlimb and identified several differences that lead to signaling deficiency, failure to retard mitosis, premature chondrocyte differentiation, and failure of dorsoventral axial asymmetry. These differences point to possible interventions to improve blastema formation and pattern formation in the froglet limb.
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