Evidence map›Paper›PMID 25062349›Full record

ArticleInternational journal of molecular sciences2014

Potential spermatogenesis recovery with bone marrow mesenchymal stem cells in an azoospermic rat model.

Deying Zhang, Xing Liu, Jinpu Peng, Dawei He, Tao Lin, Jing Zhu, Xuliang Li, Yuanyuan Zhang, Guanghui Wei

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed, 2 syntheses or guidelines pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Deying ZhangDepartment of Urology, Children's Hospital of Chongqing Medical University, No.136, Zhongshan 2 RD, Yuzhong District, Chongqing 400014, China. dzdy199@126.com.
Xing LiuDepartment of Urology, Children's Hospital of Chongqing Medical University, No.136, Zhongshan 2 RD, Yuzhong District, Chongqing 400014, China. liux_0217@163.com.
Jinpu PengDepartment of Urology, Children's Hospital of Chongqing Medical University, No.136, Zhongshan 2 RD, Yuzhong District, Chongqing 400014, China. pink-floyd@foxmail.com.
Dawei HeDepartment of Urology, Children's Hospital of Chongqing Medical University, No.136, Zhongshan 2 RD, Yuzhong District, Chongqing 400014, China. dw.he@163.com.
Tao LinDepartment of Urology, Children's Hospital of Chongqing Medical University, No.136, Zhongshan 2 RD, Yuzhong District, Chongqing 400014, China. lintao272@aliyun.com.
Jing ZhuMinistry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, China. jingzhu@cqmu.edu.cn.
Xuliang LiDepartment of Urology, Children's Hospital of Chongqing Medical University, No.136, Zhongshan 2 RD, Yuzhong District, Chongqing 400014, China. lixuliang2003@aliyun.com.
Yuanyuan ZhangDepartment of Urology, Children's Hospital of Chongqing Medical University, No.136, Zhongshan 2 RD, Yuzhong District, Chongqing 400014, China. yzhang@wakehealth.edu.
Guanghui WeiDepartment of Urology, Children's Hospital of Chongqing Medical University, No.136, Zhongshan 2 RD, Yuzhong District, Chongqing 400014, China. ghwei@cqmu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Non-obstructive azoospermia is the most challenging type of male infertility. Stem cell based therapy provides the potential to enhance the recovery of spermatogenesis following cancer therapy. Bone marrow-derived mesenchymal stem cells (BMSCs) possess the potential to differentiate or trans-differentiate into multi-lineage cells, secrete paracrine factors to recruit the resident stem cells to participate in tissue regeneration, or fuse with the local cells in the affected region. In this study, we tested whether spermatogenically-induced BMSCs can restore spermatogenesis after administration of an anticancer drug. Allogeneic BMSCs were co-cultured in conditioned media derived from cultured testicular Sertoli cells in vitro, and then induced stem cells were transplanted into the seminiferous tubules of a busulfan-induced azoospermatic rat model for 8 weeks. The in vitro induced BMSCs exhibited specific spermatogonic gene and protein markers, and after implantation the donor cells survived and located at the basement membranes of the recipient seminiferous tubules, in accordance with what are considered the unique biological characteristics of spermatogenic stem cells. Molecular markers of spermatogonial stem cells and spermatogonia (Vasa, Stella, SMAD1, Dazl, GCNF, HSP90α, integrinβ1, and c-kit) were expressed in the recipient testis tissue. No tumor mass, immune response, or inflammatory reaction developed. In conclusion, BMSCs might provide the potential to trans-differentiate into spermatogenic-like-cells, enhancing endogenous fertility recovery. The present study indicates that BMSCs might offer alternative treatment for the patients with azoospermatic infertility after cancer chemotherapy.

Indexed as

AnimalsAntineoplastic AgentsAzoospermiaBone Marrow CellsBusulfanCells, CulturedCell TransdifferentiationCoculture TechniquesDEAD-box RNA HelicasesDisease Models, AnimalMaleMesenchymal Stem CellsRatsRats, Sprague-DawleyRepressor ProteinsSertoli CellsAntineoplastic AgentsBusulfanDEAD-box RNA HelicasesRepressor Proteins

Identifiers

PMID25062349
PMCPMC4159785

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.