ArticleAIDS (London, England)2014
Renal epithelial cells produce and spread HIV-1 via T-cell contact.
Article in AIDS (London, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 36 citations in OpenAlex.
- A Contemporary Multidimensional Insight into the Clinical and Pathological Presentation of Urological Conditions Associated with HIV: A Narrative Review.Tropical medicine and infectious disease · 2025Review
- HIV-1 exploits LBPA-dependent intraepithelial trafficking for productive infection of human intestinal mucosa.PLoS pathogens · 2024Article
- Longitudinal analysis of viral dynamics in HIV+-to-HIV+ HOPE Act kidney-transplant recipients.The Journal of clinical investigation · 2024Article
- Kidney Disease Modeling with Organoids and Organs-on-Chips.Annual review of biomedical engineering · 2024Review
- Characterization of immortalized human podocytes infected with lentivirus as anAmerican journal of clinical and experimental immunology · 2024Article
- HIV-1 infection of renal epithelial cells: 30 years of evidence from transgenic animal models, human studies and in vitro experiments.Retrovirology · 2023Review
- A Systematic Review Exploring the Range of Renal Complications of Human Immunodeficiency Virus.Cureus · 2023Review
- Tubular-specific expression of HIV protein Vpr leads to severe tubulointerstitial damage accompanied by progressive fibrosis and cystic development.Kidney international · 2023Article
- HIV at 40: kidney disease in HIV treatment, prevention, and cure.Kidney international · 2022Review
- Establishment, Persistence, and Reactivation of Latent HIV-1 Infection in Renal Epithelial Cells.Journal of virology · 2022Article
- Similarities and Differences between COVID-19-Associated Nephropathy and HIV-Associated Nephropathy.Kidney diseases (Basel, Switzerland) · 2022Review
- Lactate Suppresses Retroviral Transduction in Cervical Epithelial Cells through DNA-PKcs Modulation.International journal of molecular sciences · 2021Article
- Urinary biomarkers of early renal injury in antiretroviral-naïve HIV-positive persons in Shanghai, China: comparison with the general population.HIV medicine · 2021Article
- Low expression of HIV genes in podocytes accelerates the progression of diabetic kidney disease in mice.Kidney international · 2021Article
- Potential for Virus Endogenization in Humans through Testicular Germ Cell Infection: the Case of HIV.Journal of virology · 2020Article
- Article
- Proliferation of HIV-infected renal epithelial cells following virus acquisition from infected macrophages.AIDS (London, England) · 2020Article
- Detection and molecular characterization of urinary tract HIV-1 populations.Annals of clinical microbiology and antimicrobials · 2019Article
- Tonsillitis exacerbates renal injury in IgA nephropathy through promoting Th22 cells chemotaxis.International urology and nephrology · 2018Article
- Molecular Mechanisms of Injury in HIV-Associated Nephropathy.Frontiers in medicine · 2018Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
objectivesIncreasing evidence supports the role of the kidney as a reservoir for HIV-1. In-vitro co-cultivation of HIV-infected T cells with renal tubule epithelial (RTE) cells results in virus transfer to the latter, whereas cell-free virus infection is inefficient. We further characterized the fate of HIV-1 after it is internalized in renal epithelial cells.
methodsPrimary or immortalized CD4 cells were infected with a green fluorescent protein (GFP)-expressing replication competent HIV-1. HIV-1 transfer from T cells to RTE cells was carried out in a co-culture system and evaluated by fluorescence-activated cell sorting analysis. HIV-1 integration in renal cells was evaluated by Alu-PCR and the production of infectious particles was assessed by p24-ELISA and TZM-bl assay. HIV-infected renal cells were used as donor cells in a co-culture system to evaluate their ability to transfer the virus back to T cells.
resultsRenal cells become productively infected by HIV-1 and multiple copies of HIV-1 can be transferred from infected T cells to renal cells. Two separate cell populations were identified among infected renal cells based on reporter gene GFP expression level (low vs. high), only the high showing sensitivity to azidothymidine and ritonavir. Co-cultivation of HIV-1-infected renal cells with noninfected T cells resulted in HIV-1 transmission to T cells, supporting bidirectional exchange of virus between T cells and kidney-derived cells. Persistent expression and generation of infectious virus in renal cells required HIV integration.
conclusionThese results support the kidney as a potential reservoir where virus is exchanged between interstitial T cells and RTE cells.
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