Evidence map›Paper›PMID 25061689›Full record

ArticleJournal of inorganic biochemistry2014

Cellular uptake and cytotoxicity of a near-IR fluorescent corrole-TiO2 nanoconjugate.

Carl M Blumenfeld, Bryce F Sadtler, G Esteban Fernandez, Lily Dara, Cathie Nguyen, Felix Alonso-Valenteen, Lali Medina-Kauwe, Rex A Moats, Nathan S Lewis, Robert H Grubbs and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of inorganic biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 16 citations in OpenAlex.

  1. Fighting Cancer with Corroles.Chemical reviews · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 1 country.

Carl M BlumenfeldBeckman Institute, California Institute of Technology, Pasadena, CA 91125, USA; Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Bryce F SadtlerDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
G Esteban FernandezCellular Imaging Core, The Saban Research Institute, Children's Hospital Los Angeles, Keck School of Medicine of USC, Los Angeles, CA 90027, USA.
Lily DaraDivision of Gastrointestinal and Liver Diseases, Department of Medicine, Keck School of Medicine of USC, Los Angeles, CA 90089, USA.
Cathie NguyenDepartment of Biological Sciences, Cedars-Sinai Medical Center, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90068, USA; Translational Biomedical Imaging Laboratory, Department of Radiology, The Saban Research Institute, Children's Hospital Los Angeles, Keck School of Medicine of USC, Los Angeles, CA 90027, USA.
Felix Alonso-ValenteenDepartment of Biological Sciences, Cedars-Sinai Medical Center, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90068, USA.
Lali Medina-KauweDepartment of Biological Sciences, Cedars-Sinai Medical Center, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90068, USA.
Rex A MoatsTranslational Biomedical Imaging Laboratory, Department of Radiology, The Saban Research Institute, Children's Hospital Los Angeles, Keck School of Medicine of USC, Los Angeles, CA 90027, USA.
Nathan S LewisBeckman Institute, California Institute of Technology, Pasadena, CA 91125, USA; Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Robert H GrubbsDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Harry B GrayBeckman Institute, California Institute of Technology, Pasadena, CA 91125, USA; Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA. Electronic address: hbgray@caltech.edu.
Karn SorasaeneeBeckman Institute, California Institute of Technology, Pasadena, CA 91125, USA; Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA; Translational Biomedical Imaging Laboratory, Department of Radiology, The Saban Research Institute, Children's Hospital Los Angeles, Keck School of Medicine of USC, Los Angeles, CA 90027, USA. Electronic address: ksorasaenee@chla.usc.edu.
California Institute of Technology · USChildren's Hospital of Los Angeles · USCedars-Sinai Medical Center · USKeck Hospital of USC · USUniversity of California, Los Angeles · US

Funding

ELECTRON-TRANSFER PROCESSES IN IRON AND COPPER PROTEINSR01DK019038 · NIDDK · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI GRAY, HARRY B, WINKLER, JAY R · 1986 to 2023
$7.0M
REAGENTS FOR ORGANIC SYNTHESIS BASED ON TRANSITION METALR01GM031332 · NIGMS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI GRUBBS, ROBERT HOWARD · 1985 to 2016
$6.1M
Protein-DNA Drug Carriers for Tumor TargetingR01CA129822 · NCI · CEDARS-SINAI MEDICAL CENTER · PI MEDINA-KAUWE, LALI K · 2009 to 2020
$3.0M
NCI NIH HHS R01 CA129822NIDDK NIH HHS R01 DK019038NIGMS NIH HHS R01 GM031332
6 · The paper itself

Abstract

We are investigating the biological and biomedical imaging roles and impacts of fluorescent metallocorrole-TiO2 nanoconjugates as potential near-infrared optical contrast agents in vitro in cancer and normal cell lines. The TiO2 nanoconjugate labeled with the small molecule 2,17-bis(chlorosulfonyl)-5,10,15-tris(pentafluorophenyl)corrolato aluminum(III) (1-Al-TiO2) was prepared. The nanoparticle 1-Al-TiO2 was characterized by transmission electron microscopy (TEM) and integrating-sphere electronic absorption spectroscopy. TEM images of three different samples of TiO2 nanoparticles (bare, H2O2 etched, and 1-Al functionalized) showed similarity in shapes and sizes with an average diameter of 29nm for 1-Al-TiO2. Loading of 1-Al on the TiO2 surfaces was determined to be ca. 20-40mg 1-Al/g TiO2. Confocal fluorescence microscopy (CFM) studies of luciferase-transfected primary human glioblastoma U87-Luc cells treated with the nanoconjugate 1-Al-TiO2 as the contrast agent in various concentrations were performed. The CFM images revealed that 1-Al-TiO2 was found inside the cancer cells even at low doses (0.02-2μg/mL) and localized in the cytosol. Bioluminescence studies of the U87-Luc cells exposed to various amounts of 1-Al-TiO2 showed minimal cytotoxic effects even at higher doses (2-2000μg/mL) after 24h. A similar observation was made using primary mouse hepatocytes (PMH) treated with 1-Al-TiO2 at low doses (0.0003-3μg/mL). Longer incubation times (after 48 and 72h for U87-Luc) and higher doses (>20μg/mL 1-Al-TiO2 for U87-Luc and >3μg/mL 1-Al-TiO2 for PMH) showed decreased cell viability.

Indexed as

NanostructuresAnimalsCells, CulturedHepatocytesHumansMiceMicroscopy, Electron, TransmissionMicroscopy, FluorescencePorphyrinsSpectroscopy, Near-InfraredTitaniumcorrolePorphyrinsTitaniumtitanium dioxideCellular uptakeCorroleCytotoxic effectFluorescenceNanoconjugateTiO(2) nanoparticle

Identifiers

PMID25061689
PMCPMC4998051
OpenAlexW2026262941

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.