Evidence map›Paper›PMID 25061043›Full record

ArticleMicrobiology (Reading, England)2014

Sro7 and Sro77, the yeast homologues of the Drosophila lethal giant larvae (Lgl), regulate cell proliferation via the Rho1-Tor1 pathway.

Liang-Chun Liou, Qun Ren, Qiuqiang Gao, Zhaojie Zhang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology (Reading, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Liang-Chun LiouDepartment of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA.
Qun RenDepartment of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA.
Qiuqiang GaoState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China.
Zhaojie ZhangDepartment of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA.
University of Wyoming · USEast China University of Science and Technology · CN

Funding

NEUROSCIENCE CORE CENTERP30GM103398 · NIGMS · UNIVERSITY OF WYOMING · PI FLYNN, FRANCIS W · 2012 to 2015
$3.9M
NIGMS NIH HHS P30 GM103398
6 · The paper itself

Abstract

Saccharomyces cerevisiae Sro7 and Sro77 are homologues of the Drosophila tumour suppressor lethal giant larvae (Lgl), which regulates cell polarity in Drosophila epithelial cells. Here, we showed that double mutation of SRO7/SRO77 was defective in colony growth. The colony of the SRO7/SRO77 double deletion was much smaller than the WT and appeared to be round with a smooth surface, compared with the WT. Analysis using transmission electron microscopy revealed multiple defects of the colony cells, including multiple budding, multiple nuclei, cell lysis and dead cells, suggesting that the double deletion caused defects in cell polarity and cell wall integrity (CWI). Overexpression of RHO1, one of the central regulators of cell polarity and CWI, fully recovered the sro7Δ/sro77Δ phenotype. We further demonstrated that sro7Δ/sro77Δ caused a decrease of the GTP-bound, active Rho1, which in turn caused an upregulation of TOR1. Deletion of TOR1 in sro7Δ/sro77Δ (sro7Δ/sro77Δ/tor1Δ) recovered the cell growth and colony morphology, similar to WT. Our results suggested that the tumour suppressor homologue SRO7/SRO77 regulated cell proliferation and yeast colony development via the Rho1-Tor1 pathway.

Indexed as

Gene Expression Regulation, FungalAdaptor Proteins, Signal TransducingCell PolarityCell WallMicroscopy, Electron, TransmissionMutationPhosphatidylinositol 3-KinasesPhosphoric Monoester Hydrolasesrho GTP-Binding ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsAdaptor Proteins, Signal TransducingINP53 protein, S cerevisiaePhosphatidylinositol 3-KinasesPhosphoric Monoester HydrolasesRHO1 protein, S cerevisiaerho GTP-Binding ProteinsSaccharomyces cerevisiae ProteinsSRO7 protein, S cerevisiaeTOR1 protein, S cerevisiae

Identifiers

PMID25061043
PMCPMC4178327
OpenAlexW1990283791

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.