ArticleScientific reports2014
Channelrhodopsin2 current during the action potential: "optical AP clamp" and approximation.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Photonic Nanojet-Mediated Optogenetics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Cardiac optogenetics: a decade of enlightenment.Nature reviews. Cardiology · 2021Review
- Near-infrared light driven tissue-penetrating cardiac optogenetics via upconversion nanoparticles in vivo.Biomedical optics express · 2020Article
- Light-Activated Dynamic Clamp Using iPSC-Derived Cardiomyocytes.Biophysical journal · 2018Article
- Acute Optogenetic Modulation of Cardiac Twitch Dynamics Explored Through Modeling.Journal of biomechanical engineering · 2016Article
- Light-based Approaches to Cardiac Arrhythmia Research: From Basic Science to Translational Applications.Clinical Medicine Insights. Cardiology · 2016Review
- Cardiac Optogenetics: Enhancement by All-trans-Retinal.Scientific reports · 2015Article
- Computational modeling of cardiac optogenetics: Methodology overview & review of findings from simulations.Computers in biology and medicine · 2015Review
- Optogenetic versus Electrical Stimulation of Human Cardiomyocytes: Modeling Insights.Biophysical journal · 2015Article
- "Beauty is a light in the heart": the transformative potential of optogenetics for clinical applications in cardiovascular medicine.Trends in cardiovascular medicine · 2015Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The most widely used optogenetic tool, Channelrhodopsin2 (ChR2), is both light- and voltage-sensitive. A light-triggered action potential or light-driven perturbations of ongoing electrical activity provide instant voltage feedback, shaping ChR2 current. Therefore, depending on the cell type and the light pulse duration, the typically reported voltage-clamp-measured ChR2 current traces are often not a good surrogate for the ChR2 current during optically-triggered action potentials. We discuss two experimental methods to reveal ChR2 current during an action potential: an "optical AP clamp" and its approximation employing measured current-voltage curve for ChR2. The methods are applicable to voltage- and light-sensitive ion currents operating in excitable cells, e.g. cardiomyocytes or neurons.
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Registered trials
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