ArticleProceedings of the National Academy of Sciences of the United States of America2014
Structural basis for the extended CAP-Gly domains of p150(glued) binding to microtubules and the implication for tubulin dynamics.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Article
- TRIM Proteins and Antiviral Microtubule Reorganization: A Novel Component in Innate Immune Responses?Viruses · 2024Review
- Highlighting the hidden: monitoring the avidity-driven association of a fluorescent GABARAP tandem with microtubules in living cells.Autophagy reports · 2024Article
- Crystal structure of the kinase domain of a receptor tyrosine kinase from a choanoflagellate, Monosiga brevicollis.PloS one · 2023Article
- CLIP-170S is a microtubule +TIP variant that confers resistance to taxanes by impairing drug-target engagement.Developmental cell · 2021Article
- A novel lncRNA ARST represses glioma progression by inhibiting ALDOA-mediated actin cytoskeleton integrity.Journal of experimental & clinical cancer research : CR · 2021Article
- Posttranslational modifications of the cytoskeleton.Cytoskeleton (Hoboken, N.J.) · 2021Review
- Distinct retrograde microtubule motor sets drive early and late endosome transport.The EMBO journal · 2020Article
- The tubulin code and its role in controlling microtubule properties and functions.Nature reviews. Molecular cell biology · 2020Review
- A Combinatorial MAP Code Dictates Polarized Microtubule Transport.Developmental cell · 2020Article
- The disordered N-terminus of HDAC6 is a microtubule-binding domain critical for efficient tubulin deacetylation.The Journal of biological chemistry · 2020Article
- Conformational Flexibility of p150Biophysical journal · 2019Article
- Direct induction of microtubule branching by microtubule nucleation factor SSNA1.Nature cell biology · 2018Article
- Cooperative Accumulation of Dynein-Dynactin at Microtubule Minus-Ends Drives Microtubule Network Reorganization.Developmental cell · 2018Article
- Microtubule Tip Tracking by the Spindle and Kinetochore Protein Ska1 Requires Diverse Tubulin-Interacting Surfaces.Current biology : CB · 2017Article
- Combinatorial regulation of the balance between dynein microtubule end accumulation and initiation of directed motility.The EMBO journal · 2017Article
- TheJournal of developmental biology · 2017Article
- The B-box module of CYLD is responsible for its intermolecular interaction and cytoplasmic localization.Oncotarget · 2017Article
- Dynactin binding to tyrosinated microtubules promotes centrosome centration in C. elegans by enhancing dynein-mediated organelle transport.PLoS genetics · 2017Article
- Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
p150(glued) belongs to a group of proteins accumulating at microtubule plus ends (+TIPs). It plays a key role in initiating retrograde transport by recruiting and tethering endosomes and dynein to microtubules. p150(glued) contains an N-terminal microtubule-binding cytoskeleton-associated protein glycine-rich (CAP-Gly) domain that accelerates tubulin polymerization. Although this copolymerization is well-studied using light microscopic techniques, structural consequences of this interaction are elusive. Here, using electron-microscopic and spectroscopic approaches, we provide a detailed structural view of p150(glued) CAP-Gly binding to microtubules and tubulin. Cryo-EM 3D reconstructions of p150(glued)-CAP-Gly complexed with microtubules revealed the recognition of the microtubule surface, including tubulin C-terminal tails by CAP-Gly. These binding surfaces differ from other retrograde initiation proteins like EB1 or dynein, which could facilitate the simultaneous attachment of all accessory components. Furthermore, the CAP-Gly domain, with its basic extensions, facilitates lateral and longitudinal interactions of tubulin molecules by covering the tubulin acidic tails. This shielding effect of CAP-Gly and its basic extensions may provide a molecular basis of the roles of p150(glued) in microtubule dynamics.
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