ArticleBMC microbiology2014
Differential responses of osteoblasts and macrophages upon Staphylococcus aureus infection.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 82 citations in OpenAlex.
- Osteomyelitis.Nature reviews. Disease primers · 2026Review
- Manganese enhances macrophage bactericidal activity in mice with Staphylococcus aureus osteomyelitis via Sirt3-mitophagy axis.Nature communications · 2026Article
- Enhancement of antibiotic therapy in osteomyelitis by inhibiting talin-1-mediated bacterial internalization.Frontiers in cellular and infection microbiology · 2026Article
- A methodological approach to improve extracellular staphylococcal clearance in internalization assays using bone-associated host-cell models of orthopedic infections.Frontiers in cellular and infection microbiology · 2026Article
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- [Arthritis and osteomyelitis in childhood and adolescence-Bacterial and nonbacterial].Zeitschrift fur Rheumatologie · 2025Review
- Addressing the challenges of infectious bone defects: a review of recent advances in bifunctional biomaterials.Journal of nanobiotechnology · 2025Review
- Supramolecular discrimination and diagnosis-guided treatment of intracellular bacteria.Nature communications · 2025Article
- Dalbavancin Use in Bone and Joint Infections.Arthroplasty today · 2024Article
- Antibacterial Activity of Zinc-Doped Hydroxyapatite and Vancomycin-Loaded Gelatin Nanoparticles against IntracellularACS applied nano materials · 2024Article
- MappingMicroorganisms · 2024Article
- Shock wave assisted intracellular delivery of antibiotics against bone infection withJournal of orthopaedic translation · 2024Article
- Metabolic diversity of human macrophages: potential influence onInfection and immunity · 2024Article
- Measurement of Accumulation of Antibiotics to Staphylococcus aureus in Phagosomes of Live Macrophages.Angewandte Chemie (International ed. in English) · 2024Article
- A review on antimicrobial strategies in mitigating biofilm-associated infections on medical implants.Current research in microbial sciences · 2024Review
- Global Transcriptomic Study of Circular-RNA Expression Profile in Osteoclasts Infected by IntracellularInfection and immunity · 2023Article
- Evaluation of silver bio-functionality in a multicellularFrontiers in cellular and infection microbiology · 2023Article
- Article
- Can intracellular Staphylococcus aureus in osteomyelitis be treated using current antibiotics? A systematic review and narrative synthesis.Bone research · 2022Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundStaphylococcus aureus (S. aureus) is one of the primary causes of bone infections which are often chronic and difficult to eradicate. Bacteria like S. aureus may survive upon internalization in cells and may be responsible for chronic and recurrent infections. In this study, we compared the responses of a phagocytic cell (i.e. macrophage) to a non-phagocytic cell (i.e. osteoblast) upon S. aureus internalization.
resultsWe found that upon internalization, S. aureus could survive for up to 5 and 7 days within macrophages and osteoblasts, respectively. Significantly more S. aureus was internalized in macrophages compared to osteoblasts and a significantly higher (100 fold) level of live intracellular S. aureus was detected in macrophages compared to osteoblasts. However, the percentage of S. aureus survival after infection was significantly lower in macrophages compared to osteoblasts at post-infection days 1-6. Interestingly, macrophages had relatively lower viability in shorter infection time periods (i.e. 0.5-4 h; significant at 2 h) but higher viability in longer infection time periods (i.e. 6-8 h; significant at 8 h) compared to osteoblasts. In addition, S. aureus infection led to significant changes in reactive oxygen species production in both macrophages and osteoblasts. Moreover, infected osteoblasts had significantly lower alkaline phosphatase activity at post-infection day 7 and infected macrophages had higher phagocytosis activity compared to non-infected cells.
conclusionsS. aureus was found to internalize and survive within osteoblasts and macrophages and led to differential responses between osteoblasts and macrophages. These findings may assist in evaluation of the pathogenesis of chronic and recurrent infections which may be related to the intracellular persistence of bacteria within host cells.
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