ArticleScientific reports2014
Evaluation of transmission risks associated with in vivo replication of several high containment pathogens in a biosafety level 4 laboratory.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- The face of Ebola: changing frequency of haemorrhage in the West African compared with Eastern-Central African outbreaks.BMC infectious diseases · 2015Pooled it
- Role of Key Infectivity Parameters in the Transmission of Ebola Virus Makona in Macaques.The Journal of infectious diseases · 2022Article
- Drivers of African Filovirus (Ebola and Marburg) Outbreaks.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2022Review
- Pathogen Dose in Animal Models of Hemorrhagic Fever Virus Infections and the Potential Impact on Studies of the Immune Response.Pathogens (Basel, Switzerland) · 2021Review
- Nosocomial Transmission of Emerging Viruses via Aerosol-Generating Medical Procedures.Viruses · 2019Review
- Application of next generation sequencing technology on contamination monitoring in microbiology laboratory.Biosafety and health · 2019Article
- Modeling Ebola Virus Transmission Using Ferrets.mSphere · 2018Article
- ZMapp Reinforces the Airway Mucosal Barrier Against Ebola Virus.The Journal of infectious diseases · 2018Article
- Second International Conference on Crimean-Congo Hemorrhagic Fever.Antiviral research · 2018Article
- Animal models for filovirus infections.Zoological research · 2018Review
- Crimean-Congo Hemorrhagic Fever in Humanized Mice Reveals Glial Cells as Primary Targets of Neurological Infection.The Journal of infectious diseases · 2017Article
- Animal Models of Ebolavirus Infection.Comparative medicine · 2017Review
- Ebola Virus Shedding and Transmission: Review of Current Evidence.The Journal of infectious diseases · 2016Article
- Aerosol Transmission of Filoviruses.Viruses · 2016Review
- Effectively Communicating the Uncertainties Surrounding Ebola Virus Transmission.PLoS pathogens · 2015Article
- Ebola virus disease in nonendemic countries.Journal of the Formosan Medical Association = Taiwan yi zhi · 2015Review
- Review
- Understanding ebola virus transmission.Viruses · 2015Review
- Ocular Manifestations of Ebola Virus Disease: An Ophthalmologist's Guide to Prevent Infection and Panic.BioMed research international · 2015Review
- Ebola: Implications and Perspectives.Transactions of the American Clinical and Climatological Association · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Containment level 4 (CL4) laboratories studying biosafety level 4 viruses are under strict regulations to conduct nonhuman primate (NHP) studies in compliance of both animal welfare and biosafety requirements. NHPs housed in open-barred cages raise concerns about cross-contamination between animals, and accidental exposure of personnel to infectious materials. To address these concerns, two NHP experiments were performed. One examined the simultaneous infection of 6 groups of NHPs with 6 different viruses (Machupo, Junin, Rift Valley Fever, Crimean-Congo Hemorrhagic Fever, Nipah and Hendra viruses). Washing personnel between handling each NHP group, floor to ceiling biobubble with HEPA filter, and plexiglass between cages were employed for partial primary containment. The second experiment employed no primary containment around open barred cages with Ebola virus infected NHPs 0.3 meters from naïve NHPs. Viral antigen-specific ELISAs, qRT-PCR and TCID50 infectious assays were utilized to determine antibody levels and viral loads. No transmission of virus to neighbouring NHPs was observed suggesting limited containment protocols are sufficient for multi-viral CL4 experiments within one room. The results support the concept that Ebola virus infection is self-contained in NHPs infected intramuscularly, at least in the present experimental conditions, and is not transmitted to naïve NHPs via an airborne route.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.