Evidence map›Paper›PMID 25058879›Full record

ArticlePancreas2014

Pancreatic ductal perfusion at organ procurement enhances islet yield in human islet isolation.

Morihito Takita, Takeshi Itoh, Masayuki Shimoda, Mazhar A Kanak, Rauf Shahbazov, Faisal Kunnathodi, Michael C Lawrence, Bashoo Naziruddin, Marlon F Levy

Open access · greenAbstract readComparative StudyControlled Clinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Article in Pancreas, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Morihito Takita *Islet Cell Laboratory, Baylor Research Institute, Dallas, Texas.
Takeshi Itoh *Islet Cell Laboratory, Baylor Research Institute, Dallas, Texas.
Masayuki ShimodaIslet Cell Laboratory, Baylor Research Institute, Dallas, Texas.
Mazhar A KanakInstitute for Biomedical Studies, Baylor University, Waco, Texas.
Rauf ShahbazovIslet Cell Laboratory, Baylor Research Institute, Dallas, Texas.
Faisal KunnathodiIslet Cell Laboratory, Baylor Research Institute, Dallas, Texas.
Michael C LawrenceIslet Cell Laboratory, Baylor Research Institute, Dallas, Texas.
Bashoo NaziruddinBaylor Annette C. and Harold C. Simmons Transplant Institute, Baylor University Medical Center, Dallas, Texas.
Marlon F LevyBaylor Annette C. and Harold C. Simmons Transplant Institute, Baylor University Medical Center, Dallas, Texas.
Baylor University Medical Center · USBaylor University · US

Funding

Randomized Comparison of Ductal Preservation Solutions for Improving QuantityR21DK090513 · NIDDK · BAYLOR RESEARCH INSTITUTE · PI LEVY, MARLON FRANTZ · 2011 to 2012
$431k
NIDDK NIH HHS 1R21DK090513-019NIDDK NIH HHS R21 DK090513
6 · The paper itself

Abstract

objectivePancreas preservation is a major factor influencing the results of islet cell transplantation. This study evaluated the effects of 2 different solutions for pancreatic ductal perfusion (PDP) at organ procurement.

methodsEighteen human pancreases were assigned to 3 groups: non-PDP (control), PDP with ET-Kyoto solution, and PDP with cold storage/purification stock solution. Pancreatic islets were isolated according to the modified Ricordi method.

resultsNo significant differences in donor characteristics, including cold ischemia time, were observed between the 3 groups. All islet isolations in the PDP groups had more than 400,000 islet equivalence in total islet yield after purification, a significant increase when compared with the control (P = 0.04 and P < 0.01). The islet quality assessments, including an in vivo diabetic nude mice assay and the response of high-mobility group box protein 1 to cytokine stimulation, also showed no significant differences. The proportion of terminal deoxynucleotidyl transferase dUTP nick-end labeling-positive cells showing apoptosis in islets in the PDP groups was significantly lower than in the control group (P < 0.05).

conclusionsBoth ET-Kyoto solution and cold storage/purification stock solution are suitable for PDP and consistently resulted in isolation success. Further studies with a larger number of pancreas donors should be done to compare the effects of the PDP solutions.

Indexed as

Islets of LangerhansAdultAgedAnimalsApoptosisCell CountCells, CulturedCell SeparationCytokinesDiabetes Mellitus, ExperimentalFemaleGluconatesHeterograftsHistidineHMGB1 ProteinHumansCytokinesET-Kyoto solutionGluconatesHistidineHMGB1 ProteinHydroxyethyl Starch DerivativesOrgan Preservation SolutionsPhosphatesTrehalose

Identifiers

PMID25058879
PMCPMC4396628
OpenAlexW2095153011

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.