ArticlePloS one2014
Analyzing cold tolerance mechanism in transgenic zebrafish (Danio rerio).
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 68 citations in OpenAlex.
- Integrated miRNA-mRNA Atlas Reveals Temperature-Graded Brain Neuroendocrine Adaptation to Cold Stress in Silvery Pomfret (Biology · 2025Article
- Effect of organic selenium on performance and gill transcriptome of whiteleg shrimp Penaeus vannamei.Molecular biology reports · 2024Article
- The genomic basis and environmental correlates of local adaptation in the Atlantic horse mackerel (Evolutionary applications · 2023Article
- Article
- Selection-driven adaptation to the extreme Antarctic environment in the Emperor penguin.Heredity · 2022Article
- Advancing the protection of marine life through genomics.PLoS biology · 2022Article
- Energy consumption and intestinal microbiome disorders of yellow catfishFrontiers in physiology · 2022Article
- Efficacy of Tricaine (MS-222) and Hypothermia as Anesthetic Agents for Blocking Sensorimotor Responses in Larval Zebrafish.Frontiers in veterinary science · 2022Article
- Gene Expression Profile and Co-Expression Network of Pearl Gentian Grouper under Cold Stress by Integrating Illumina and PacBio Sequences.Animals : an open access journal from MDPI · 2021Article
- Induced Torpor as a Countermeasure for Low Dose Radiation Exposure in a Zebrafish Model.Cells · 2021Article
- Direct and pleiotropic effects of the Masou Salmon Delta-5 Desaturase transgene in F1 channel catfish (Ictalurus punctatus).Transgenic research · 2021Article
- Acute environmental temperature variation affects brain protein expression, anxiety and explorative behaviour in adult zebrafish.Scientific reports · 2021Article
- Genome-Wide Transcriptional Changes ofFrontiers in microbiology · 2021Article
- The power and limitations of gene expression pathway analyses toward predicting population response to environmental stressors.Evolutionary applications · 2020Article
- A novel group of secretory cells regulates development of the immature intestinal stem cell niche through repression of the main signaling pathways driving proliferation.Developmental biology · 2019Article
- Deficiency in the membrane protein Tmbim3a/Grinaa initiates cold-induced ER stress and cell death by activating an intrinsic apoptotic pathway in zebrafish.The Journal of biological chemistry · 2019Article
- Network of microRNA-transcriptional factor-mRNA in cold response of turbot Scophthalmus maximus.Fish physiology and biochemistry · 2019Article
- Fasting enhances cold resistance in fish through stimulating lipid catabolism and autophagy.The Journal of physiology · 2019Article
- CuChemical science · 2018Article
- Impacts of Early Life Stress on the Methylome and Transcriptome of Atlantic Salmon.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Low temperatures may cause severe growth inhibition and mortality in fish. In order to understand the mechanism of cold tolerance, a transgenic zebrafish Tg (smyd1:m3ck) model was established to study the effect of energy homeostasis during cold stress. The muscle-specific promoter Smyd1 was used to express the carp muscle form III of creatine kinase (M3-CK), which maintained enzymatic activity at a relatively low temperature, in zebrafish skeletal muscle. In situ hybridization showed that M3-CK was expressed strongly in the skeletal muscle. When exposed to 13 °C, Tg (smyd1:m3ck) fish maintained their swimming behavior, while the wild-type could not. Energy measurements showed that the concentration of ATP increased in Tg (smyd1:m3ck) versus wild-type fish at 28 °C. After 2 h at 13 °C, ATP concentrations were 2.16-fold higher in Tg (smyd1:m3ck) than in wild-type (P<0.05). At 13 °C, the ATP concentration in Tg (smyd1:m3ck) fish and wild-type fish was 63.3% and 20.0%, respectively, of that in wild-type fish at 28 °C. Microarray analysis revealed differential expression of 1249 transcripts in Tg (smyd1:m3ck) versus wild-type fish under cold stress. Biological processes that were significantly overrepresented in this group included circadian rhythm, energy metabolism, lipid transport, and metabolism. These results are clues to understanding the mechanisms underlying temperature acclimation in fish.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.