ArticleNature protocols2014
In situ simultaneous monitoring of ATP and GTP using a graphene oxide nanosheet-based sensing platform in living cells.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Optical Detection of Cellular Signals at Material Interfaces.Advanced healthcare materials · 2026Review
- Oxygen-vacancy-regulated molybdenum oxide nanosheets for a multi-phosphate discriminative colorimetric sensor array.Journal of advanced research · 2026Article
- Subcellular Tandem-Activated Signal Amplification for Spatially Precise Molecular Imaging in Mitochondria.Angewandte Chemie (International ed. in English) · 2026Article
- Dynamic Monitoring of Content Variation of Guanosine Triphosphate in Single Cells under External Stimulus with Functionalized Fluorescent/Photothermal NbChemical & biomedical imaging · 2026Article
- DNA-Based Fluorescent Nanoprobe for Cancer Cell Membrane Imaging.Molecules (Basel, Switzerland) · 2024Review
- Homogeneous luminescent quantitation of cellular guanosine and adenosine triphosphates (GTP and ATP) using QT-LucAnalytical and bioanalytical chemistry · 2023Article
- Combination of multiple nucleic acid aptamers for precision detection of tumors based on optical methods.Journal of cancer research and clinical oncology · 2023Review
- Recent progress of aptamer‒drug conjugates in cancer therapy.Acta pharmaceutica Sinica. B · 2023Review
- Catalytic nanozyme Zn/Cl-doped carbon quantum dots as ratiometric fluorescent probe for sequential on-off-on detection of riboflavin, CuScientific reports · 2022Article
- Recent Advances in Electrochemical Sensing of Hydrogen Peroxide (HNanomaterials (Basel, Switzerland) · 2022Review
- A fluorescence nanoplatform for the determination of hydrogen peroxide and adenosine triphosphate via tuning of the peroxidase-like activity of CuO nanoparticle decorated UiO-66.Mikrochimica acta · 2022Article
- Simultaneous probing of dual intracellular metabolites (ATP and paramylon) in live microalgae using graphene oxide/aptamer nanocomplex.Mikrochimica acta · 2022Article
- Article
- Ex vivo and in vivo fluorescence detection and imaging of adenosine triphosphate.Journal of nanobiotechnology · 2021Article
- Recent progress in developing fluorescent probes for imaging cell metabolites.Biomedical materials (Bristol, England) · 2021Review
- Tumor-Targeting Cholesterol-Decorated DNA Nanoflowers for Intracellular Ratiometric Aptasensing.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- Translational control of enzyme scavenger expression with toxin-induced micro RNA switches.Scientific reports · 2021Article
- Graphene Quantum Dot Oxidation Governs Noncovalent Biopolymer Adsorption.Scientific reports · 2020Article
- Nucleic-Acid Structures as Intracellular Probes for Live Cells.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- Graphene/aptamer probes for small molecule detection: from in vitro test to in situ imaging.Mikrochimica acta · 2020Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we present a detailed protocol for in situ multiple fluorescence monitoring of adenosine-5'-triphosphate (ATP) and guanosine-5'-triphosphate (GTP) in MCF-7 breast cancer cells by using graphene oxide nanosheet (GO-nS) and DNA/RNA aptamers. FAM-labeled ATP aptamer and Cy5-modified GTP aptamer are used to construct the multiple aptamer/GO-nS sensing platform through 'π-π stacking' between aptamers and GO-nS. Binding of aptamers to GO-nS guarantees the fluorescence resonance energy transfer between fluorophores and GO-nS, resulting in 'fluorescence off'. When the aptamer/GO-nS are transported inside the cells via endocytosis, the conformation of the aptamers will change on interaction with cellular ATP and GTP. On the basis of the fluorescence 'off/on' switching, simultaneous sensing and imaging of ATP and GTP in vitro and in situ have been realized through fluorescence and confocal microscopy techniques. In this protocol, we describe the synthesis of GO and GO-nS, preparation of aptamer/GO-nS platform, in vitro detection of ATP and GTP, and how to use this platform to realize intracellular ATP and GTP imaging in cultured MCF-7 cells. The preparation of GO-nS is anticipated to take 7-14 d, and assays involving microscopy imaging and MCF-7 cells culturing can be performed in 2-3 d.
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