ArticleMolecular endocrinology (Baltimore, Md.)2014
Fatty acid synthesis and oxidation in cumulus cells support oocyte maturation in bovine.
Article in Molecular endocrinology (Baltimore, Md.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers, 1 of them a synthesis that pooled it.
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Who cites it
62 citing papers in PubMed, 1 synthesis or guideline pooled it, 125 citations in OpenAlex.
- Transcriptome meta-analysis of three follicular compartments and its correlation with ovarian follicle maturity and oocyte developmental competence in cows.Physiological genomics · 2016Pooled it
- Review
- Article
- Propionic Acid Supports the Development of Oocytes Derived from Bovine Early Antral Follicles.Veterinary sciences · 2026Article
- Heat Shock Affects Amino Acid Metabolism in Bovine Cumulus Cells and Denuded Oocytes During In Vitro Maturation.Biology · 2026Article
- Bisphenols and follicle function in farm species.The Journal of reproduction and development · 2026Review
- The role of metabolic reprogramming in oocyte development: research progress and prospects.Frontiers in reproductive health · 2026Review
- Expression of New Gene Markers Regulating Protein Metabolism in Porcine Ovarian Granulosa Cells In Vitro.International journal of molecular sciences · 2025Article
- Article
- Abnormal cholesterol-cholesteryl ester metabolism impairs mouse oocyte quality during ovarian aging.Cellular & molecular biology letters · 2025Article
- Characterization of the Lipid Profile of Immature and In Vitro Matured Cat Oocytes.Molecular reproduction and development · 2025Article
- Follicular-fluid extracellular vesicles support energy metabolism of bovine oocytes, improving blastocyst development and quality†.Biology of reproduction · 2025Article
- The role of mitochondrial dysfunction in ovarian granulosa cells in polycystic ovary syndrome.Endocrine connections · 2025Review
- Involvement of Orotic Acid in Mitochondrial Activity of Ovarian Granulosa Cells and Oocyte Meiotic Maturation.International journal of molecular sciences · 2025Article
- Effect of Bovine Follicular Fluid Small Extracellular Vesicles Isolated by Ultracentrifugation and Chromatography on In Vitro Oocyte Maturation and Embryo Development.International journal of molecular sciences · 2025Article
- Follicular metabolic dysfunction, oocyte aneuploidy and ovarian aging: a review.Journal of ovarian research · 2025Review
- Follicular fluid extracellular vesicles improve bovine oocyte quality via lipid and mitochondrial modulation.Frontiers in veterinary science · 2025Article
- Tailor-made 3DFrontiers in endocrinology · 2025Article
- Potential mechanisms and therapeutic strategies for LPS-associated female fertility decline.Journal of assisted reproduction and genetics · 2024Review
- Granulosa cell metabolism at ovulation correlates with oocyte competence and is disrupted by obesity and aging.Human reproduction (Oxford, England) · 2024Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oocyte meiotic maturation requires energy from various substrates including glucose, amino acids, and lipids. Mitochondrial fatty acid (FA) β-oxidation (FAO) in the oocyte is required for meiotic maturation, which is accompanied by differential expression of numerous genes involved in FAs metabolism in surrounding cumulus cells (CCs) in vivo. The objective was to elucidate components involved in FAs metabolism in CCs during oocyte maturation. Twenty-seven genes related to lipogenesis, lipolysis, FA transport, and FAO were chosen from comparative transcriptome analysis of bovine CCs before and after maturation in vivo. Using real-time PCR, 22 were significantly upregulated at different times of in vitro maturation (IVM) in relation to oocyte meiosis progression from germinal vesicle breakdown to metaphase-II. Proteins FA synthase, acetyl-coenzyme-A carboxylase, carnitine palmitoyltransferase, perilipin 2, and FA binding protein 3 were detected by Western blot and immunolocalized to CCs and oocyte cytoplasm, with FA binding protein 3 concentrated around oocyte chromatin. By mass spectrometry, CCs lipid profiling was shown to be different before and after IVM. FAO inhibitors etomoxir and mildronate dose-dependently decreased the oocyte maturation rate in vitro. In terms of viability, cumulus enclosed oocytes were more sensitive to etomoxir than denuded oocytes. In CCs, etomoxir (150 μM) led to downregulation of lipogenesis genes and upregulated lipolysis and FAO genes. Moreover, the number of lipid droplets decreased, whereas several lipid species were more abundant compared with nontreated CCs after IVM. In conclusion, FAs metabolism in CCs is important to maintain metabolic homeostasis and may influence meiosis progression and survival of enclosed oocytes.
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