ArticlePloS one2014
The growth and survival of Mycobacterium smegmatis is enhanced by co-metabolism of atmospheric H2.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 72 citations in OpenAlex.
- Atmospheric hydrogen consumption is regulated by glycerol-mediated catabolite repression in mycobacteria.mSystems · 2026Article
- Search for the role of a Huc-type [NiFe]-hydrogenase of the soil thermophileFrontiers in microbiology · 2026Article
- ATP synthesis driven by atmospheric hydrogen concentrations.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Metabolism of CO and H2 by pioneer bacteria in volcanic soils and the phyllosphere.The ISME journal · 2025Review
- Trace gas oxidation sustains energy needs of a thermophilic archaeon at suboptimal temperatures.Nature communications · 2024Article
- Carbon amendments in soil microcosms induce uneven response on H2 oxidation activity and microbial community composition.FEMS microbiology ecology · 2023Article
- Molecular hydrogen in seawater supports growth of diverse marine bacteria.Nature microbiology · 2023Article
- Article
- Microbial oxidation of atmospheric trace gases.Nature reviews. Microbiology · 2022Review
- Sensitivity of soil hydrogen uptake to natural and managed moisture dynamics in a semiarid urban ecosystem.PeerJ · 2022Article
- Multiple energy sources and metabolic strategies sustain microbial diversity in Antarctic desert soils.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Chemosynthetic and photosynthetic bacteria contribute differentially to primary production across a steep desert aridity gradient.The ISME journal · 2021Article
- Article
- Trace gas oxidizers are widespread and active members of soil microbial communities.Nature microbiology · 2021Article
- Hydrogen-Oxidizing Bacteria Are Abundant in Desert Soils and Strongly Stimulated by Hydration.mSystems · 2020Article
- A widely distributed hydrogenase oxidises atmospheric HThe ISME journal · 2020Article
- The thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV oxidizes subatmospheric HThe ISME journal · 2020Article
- Molecular Hydrogen Metabolism: a Widespread Trait of Pathogenic Bacteria and Protists.Microbiology and molecular biology reviews : MMBR · 2020Review
- Impact of Oxygen Supply and Scale Up onFrontiers in bioengineering and biotechnology · 2020Article
- Two uptake hydrogenases differentially interact with the aerobic respiratory chain during mycobacterial growth and persistence.The Journal of biological chemistry · 2019Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
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Abstract
The soil bacterium Mycobacterium smegmatis is able to scavenge the trace concentrations of H2 present in the atmosphere, but the physiological function and importance of this activity is not understood. We have shown that atmospheric H2 oxidation in this organism depends on two phylogenetically and kinetically distinct high-affinity hydrogenases, Hyd1 (MSMEG_2262-2263) and Hyd2 (MSMEG_2720-2719). In this study, we explored the effect of deleting Hyd2 on cellular physiology by comparing the viability, energetics, transcriptomes, and metabolomes of wild-type vs. Δhyd2 cells. The long-term survival of the Δhyd2 mutant was significantly reduced compared to the wild-type. The mutant additionally grew less efficiently in a range of conditions, most notably during metabolism of short-chain fatty acids; there was a twofold reduction in growth rate and growth yield of the Δhyd2 strain when acetate served as the sole carbon source. Hyd1 compensated for loss of Hyd2 when cells were grown in a high H2 atmosphere. Analysis of cellular parameters showed that Hyd2 was not necessary to generate the membrane potential, maintain intracellular pH homeostasis, or sustain redox balance. However, microarray analysis indicated that Δhyd2 cells were starved for reductant and compensated by rewiring central metabolism; transcripts encoding proteins responsible for oxidative decarboxylation pathways, the urea cycle, and ABC transporter-mediated import were significantly more abundant in the Δhyd2 mutant. Metabolome profiling consistently revealed an increase in intracellular amino acids in the Δhyd2 mutant. We propose that atmospheric H2 oxidation has two major roles in mycobacterial cells: to generate reductant during mixotrophic growth and to sustain the respiratory chain during dormancy.
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Registered trials
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