Evidence map›Paper›PMID 25058452›Full record

ArticleNature communications2014

Arrayed lipid bilayer chambers allow single-molecule analysis of membrane transporter activity.

Rikiya Watanabe, Naoki Soga, Daishi Fujita, Kazuhito V Tabata, Lisa Yamauchi, Soo Hyeon Kim, Daisuke Asanuma, Mako Kamiya, Yasuteru Urano, Hiroaki Suga and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.

0numbers the graph read from it
0cells of the map it votes in
46citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

46 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Substrate-supported Model Membrane as a Versatile Analytical/Biosensing Platform.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Rikiya Watanabe1] Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan [2] PRESTO, JST, Bunkyo-ku, Tokyo 113-8656, Japan [3] CREST, JST, Bunkyo-ku, Tokyo 113-8656, Japan [4].
Naoki Soga1] Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan [2].
Daishi Fujita1] Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan [2].
Kazuhito V Tabata1] Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan [2] PRESTO, JST, Bunkyo-ku, Tokyo 113-8656, Japan [3] CREST, JST, Bunkyo-ku, Tokyo 113-8656, Japan.
Lisa Yamauchi1] Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan [2] CREST, JST, Bunkyo-ku, Tokyo 113-8656, Japan.
Soo Hyeon Kim1] CREST, JST, Bunkyo-ku, Tokyo 113-8656, Japan [2] Institute of Industrial Science, The University of Tokyo, Meguro-ku, Tokyo 153-8505, Japan.
Daisuke AsanumaLaboratory of Neurobiology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
Mako KamiyaLaboratory of Chemical Biology and Molecular Imaging, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
Yasuteru UranoLaboratory of Chemical Biology and Molecular Imaging, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
Hiroaki Suga1] CREST, JST, Bunkyo-ku, Tokyo 113-8656, Japan [2] Department of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan.
Hiroyuki Noji1] Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan [2] CREST, JST, Bunkyo-ku, Tokyo 113-8656, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nano- to micron-size reaction chamber arrays (femtolitre chamber arrays) have facilitated the development of sensitive and quantitative biological assays, such as single-molecule enzymatic assays, digital PCR and digital ELISA. However, the versatility of femtolitre chamber arrays is limited to reactions that occur in aqueous solutions. Here we report an arrayed lipid bilayer chamber system (ALBiC) that contains sub-million femtolitre chambers, each sealed with a stable 4-μm-diameter lipid bilayer membrane. When reconstituted with a limiting amount of the membrane transporter proteins α-hemolysin or F0F1-ATP synthase, the chambers within the ALBiC exhibit stochastic and quantized transporting activities. This demonstrates that the single-molecule analysis of passive and active membrane transport is achievable with the ALBiC system. This new platform broadens the versatility of femtolitre chamber arrays and paves the way for novel applications aimed at furthering our mechanistic understanding of membrane proteins' function.

Indexed as

Bacterial ToxinsBiological Transport, ActiveFluorescent DyesHemolysin ProteinsHydrazinesLipid BilayersMembrane Transport ProteinsMicrotechnologyMolecular BiologyNanotechnologyPhotobleachingProton-Translocating ATPasesAlexa 488 hydrazideBacterial ToxinsFluorescent DyesHemolysin ProteinsHydrazinesLipid BilayersMembrane Transport ProteinsProton-Translocating ATPasesstaphylococcal alpha-toxin

Identifiers

PMID25058452
PMCPMC4124872

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.