ArticleBiochimica et biophysica acta2014
Engineered biosynthesis of bacteriochlorophyll b in Rhodobacter sphaeroides.
Article in Biochimica et biophysica acta, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Function, Structure, and Regulation of Nitrogen Fixation-like Metalloproteins for Nitrogen, Energy, Carbon, and Sulfur Metabolism.Chemical reviews · 2026Review
- The structural chemistry and biosynthesis of chlorophylls.RSC chemical biology · 2026Review
- Natural pigments derived from plants and microorganisms: classification, biosynthesis, and applications.Plant biotechnology journal · 2025Review
- Globally distributed Myxococcota with photosynthesis gene clusters illuminate the origin and evolution of a potentially chimeric lifestyle.Nature communications · 2023Article
- ChlorophyllBiology · 2023Article
- The role of the γ subunit in the photosystem of the lowest-energy phototrophs.The Biochemical journal · 2022Article
- Adaptation of Cyanobacteria to the Endolithic Light Spectrum in Hyper-Arid Deserts.Microorganisms · 2022Article
- Controlling synthetic membraneless organelles by a red-light-dependent singlet oxygen-generating protein.Nature communications · 2022Article
- The terminal enzymes of (bacterio)chlorophyll biosynthesis.Royal Society open science · 2022Review
- Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes.Photosynthesis research · 2021Article
- Unfolding pathway and intermolecular interactions of the cytochrome subunit in the bacterial photosynthetic reaction center.Biochimica et biophysica acta. Bioenergetics · 2020Article
- Engineering of B800 bacteriochlorophyll binding site specificity in the Rhodobacter sphaeroides LH2 antenna.Biochimica et biophysica acta. Bioenergetics · 2019Article
- A paralog of a bacteriochlorophyll biosynthesis enzyme catalyzes the formation of 1,2-dihydrocarotenoids in green sulfur bacteria.The Journal of biological chemistry · 2018Article
- Electron-Transfer Secondary Reaction Matrices for MALDI MS Analysis of Bacteriochlorophyll a in Rhodobacter sphaeroides and Its Zinc and Copper Analogue Pigments.Journal of the American Society for Mass Spectrometry · 2017Article
- Absence of the cbb3 Terminal Oxidase Reveals an Active Oxygen-Dependent Cyclase Involved in Bacteriochlorophyll Biosynthesis in Rhodobacter sphaeroides.Journal of bacteriology · 2016Article
- Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina.Journal of bacteriology · 2016Article
- Rhodobacter sphaeroides mutants overexpressing chlorophyllide a oxidoreductase of Blastochloris viridis elucidate functions of enzymes in late bacteriochlorophyll biosynthetic pathways.Scientific reports · 2015Article
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2 authors at 1 institution in 1 country.
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Abstract
Bacteriochlorophyll b has the most red-shifted absorbance maximum of all naturally occurring photopigments. It has a characteristic ethylidene group at the C8 position in place of the more common ethyl group, the product of a C8-vinyl reductase, which is carried by the majority of chlorophylls and bacteriochlorophylls used in photosynthesis. The subsequent and first step exclusive to bacteriochlorophyll biosynthesis, the reduction of the C7=C8 bond, is catalyzed by chlorophyllide oxidoreductase. It has been demonstrated that the enzyme from bacteriochlorophyll a-utilizing bacteria can catalyze the formation of compounds carrying an ethyl group at C8 from both ethyl- and vinyl-carrying substrates, indicating a surprising additional C8-vinyl reductase function, while the enzyme from organisms producing BChl b could only catalyze C7=C8 reduction with a vinyl substrate, but this product carried an ethylidene group at the C8 position. We have replaced the native chlorophyllide oxidoreductase-encoding genes of Rhodobacter sphaeroides with those from Blastochloris viridis, but the switch from bacteriochlorophyll a to b biosynthesis is only detected when the native conventional C8-vinyl reductase is absent. We propose a non-enzymatic mechanism for ethylidene group formation based on the absence of cellular C8-vinyl reductase activity.
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