ArticlePLoS neglected tropical diseases2014
Identification of Giardia lamblia DHHC proteins and the role of protein S-palmitoylation in the encystation process.
Article in PLoS neglected tropical diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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14 citing papers in PubMed, 24 citations in OpenAlex.
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- Application of Proteomics to the Study of the Therapeutics and Pathogenicity ofDiagnostics (Basel, Switzerland) · 2022Review
- A Detailed Gene Expression Map ofGenes · 2021Article
- A novel yeast-based high-throughput method for the identification of protein palmitoylation inhibitors.Open biology · 2021Article
- Staging Encystation Progression inFrontiers in cell and developmental biology · 2021Article
- Identification of Conserved Candidate Vaccine Antigens in the Surface Proteome of Giardia lamblia.Infection and immunity · 2019Article
- The dual roles of RPE65 S-palmitoylation in membrane association and visual cycle function.Scientific reports · 2019Article
- Protein Palmitoylation Plays an Important Role inMolecular & cellular proteomics : MCP · 2018Article
- Membrane-Associated Proteins inGenes · 2018Review
- Subcellular localisation of FLAG tagged enzymes of the dynamic protein S-palmitoylation cycle of Trypanosoma cruzi epimastigotes.Memorias do Instituto Oswaldo Cruz · 2018Article
- Dynamic protein S-palmitoylation mediates parasite life cycle progression and diverse mechanisms of virulence.Critical reviews in biochemistry and molecular biology · 2017Review
- Protein S-palmitoylation in cellular differentiation.Biochemical Society transactions · 2017Review
- Progress toward Understanding Protein S-acylation: Prospective in Plants.Frontiers in plant science · 2017Review
- Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein S-palmitoylation, a hydrophobic post-translational modification, is performed by protein acyltransferases that have a common DHHC Cys-rich domain (DHHC proteins), and provides a regulatory switch for protein membrane association. In this work, we analyzed the presence of DHHC proteins in the protozoa parasite Giardia lamblia and the function of the reversible S-palmitoylation of proteins during parasite differentiation into cyst. Two specific events were observed: encysting cells displayed a larger amount of palmitoylated proteins, and parasites treated with palmitoylation inhibitors produced a reduced number of mature cysts. With bioinformatics tools, we found nine DHHC proteins, potential protein acyltransferases, in the Giardia proteome. These proteins displayed a conserved structure when compared to different organisms and are distributed in different monophyletic clades. Although all Giardia DHHC proteins were found to be present in trophozoites and encysting cells, these proteins showed a different intracellular localization in trophozoites and seemed to be differently involved in the encystation process when they were overexpressed. dhhc transgenic parasites showed a different pattern of cyst wall protein expression and yielded different amounts of mature cysts when they were induced to encyst. Our findings disclosed some important issues regarding the role of DHHC proteins and palmitoylation during Giardia encystation.
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