ArticlePLoS genetics2014
Wnt signaling interacts with bmp and edn1 to regulate dorsal-ventral patterning and growth of the craniofacial skeleton.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 51 citations in OpenAlex.
- A cross-tissue physicochemical causal chain underlying vertebrate mandibular morphogenesis.Science advances · 2026Article
- Dkk1 inhibition restores mandibular growth in an achondroplasia mouse model.Biology open · 2026Article
- Analysis of cranial tenocyte heterogeneity reveals a role for Wnt signaling in tendon attachments.Development (Cambridge, England) · 2026Article
- Klf5a in Endoderm Promotes Pharyngeal Cartilage Morphogenesis.International journal of molecular sciences · 2025Article
- Nkx2.7 is a conserved regulator of craniofacial development.Nature communications · 2025Article
- A potential role of Fgf3 for epibranchial formation in zebrafish.Frontiers in cell and developmental biology · 2025Article
- Holoprosencephaly and cyclopia inAnimal cells and systems · 2025Article
- Investigating possible shared single nucleotide polymorphisms in isolated oral cleft and non-cleft facial morphology.Frontiers in dental medicine · 2025Article
- Transforming growth factor beta signaling and craniofacial development: modeling human diseases in zebrafish.Frontiers in cell and developmental biology · 2024Review
- Endothelin signaling in development.Development (Cambridge, England) · 2023Review
- Characterising the Effect of Wnt/β-Catenin Signalling on Melanocyte Development and Patterning: Insights from Zebrafish (International journal of molecular sciences · 2023Article
- Ectodermal Wnt signaling, cell fate determination, and polarity of the skate gill arch skeleton.eLife · 2023Article
- Post-transcriptional regulation in cranial neural crest cells expands developmental potential.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Genetic variants in bone morphogenetic proteins signaling pathway might be involved in palatal rugae phenotype in humans.Scientific reports · 2021Article
- MicroRNA-1 affects the development of the neural crest and craniofacial skeleton via the mitochondrial apoptosis pathway.Experimental and therapeutic medicine · 2021Article
- Synergistic roles of Wnt modulators R-spondin2 and R-spondin3 in craniofacial morphogenesis and dental development.Scientific reports · 2021Article
- Review
- Notch signaling: Its essential roles in bone and craniofacial development.Genes & diseases · 2021Review
- Review: The Role of Wnt/β-Catenin Signalling in Neural Crest Development in Zebrafish.Frontiers in cell and developmental biology · 2021Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Craniofacial development requires signals from epithelia to pattern skeletogenic neural crest (NC) cells, such as the subdivision of each pharyngeal arch into distinct dorsal (D) and ventral (V) elements. Wnt signaling has been implicated in many aspects of NC and craniofacial development, but its roles in D-V arch patterning remain unclear. To address this we blocked Wnt signaling in zebrafish embryos in a temporally-controlled manner, using transgenics to overexpress a dominant negative Tcf3, (dntcf3), (Tg(hsp70I:tcf3-GFP), or the canonical Wnt inhibitor dickkopf1 (dkk1), (Tg(hsp70i:dkk1-GFP) after NC migration. In dntcf3 transgenics, NC cells in the ventral arches of heat-shocked embryos show reduced proliferation, expression of ventral patterning genes (hand2, dlx3b, dlx5a, msxe), and ventral cartilage differentiation (e.g. lower jaws). These D-V patterning defects resemble the phenotypes of zebrafish embryos lacking Bmp or Edn1 signaling, and overexpression of dntcf3 dramatically reduces expression of a subset of Bmp receptors in the arches. Addition of ectopic BMP (or EDN1) protein partially rescues ventral development and expression of dlx3b, dlx5a, and msxe in Wnt signaling-deficient embryos, but surprisingly does not rescue hand2 expression. Thus Wnt signaling provides ventralizing patterning cues to arch NC cells, in part through regulation of Bmp and Edn1 signaling, but independently regulates hand2. Similarly, heat-shocked dkk1+ embryos exhibit ventral arch reductions, but also have mandibular clefts at the ventral midline not seen in dntcf3+ embryos. Dkk1 is expressed in pharyngeal endoderm, and cell transplantation experiments reveal that dntcf3 must be overexpressed in pharyngeal endoderm to disrupt D-V arch patterning, suggesting that distinct endodermal roles for Wnts and Wnt antagonists pattern the developing skeleton.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.