ArticlePloS one2014
Application of detergents or high hydrostatic pressure as decellularization processes in uterine tissues and their subsequent effects on in vivo uterine regeneration in murine models.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
61 citing papers in PubMed, 2 syntheses or guidelines pooled it, 140 citations in OpenAlex.
- Recent Trends in Tissue Engineering Strategies for Uterine Endometrial Regeneration: a Systematic Review.Reproductive sciences (Thousand Oaks, Calif.) · 2026Pooled it
- Bioengineering trends in female reproduction: a systematic review.Human reproduction update · 2022Pooled it
- Experimental Analysis and Modeling Study of Impedance Changes in Decellularized and Recellularized Peripheral Nerves.Bioengineering (Basel, Switzerland) · 2026Article
- Fabrication and characterization of decellularized human endometrial hydrogel as a scaffold for in vitro embryo implantation: An experimental study.International journal of reproductive biomedicine · 2026Article
- Toward human uterus tissue engineering: Uterine decellularization in a non-human primate species.Acta obstetricia et gynecologica Scandinavica · 2025Article
- Standardizing decellularization protocols for optimized uterine tissue bioengineering.Regenerative therapy · 2025Review
- Uterine Repair Mechanisms Are Potentiated by Mesenchymal Stem Cells and Decellularized Tissue Grafts Through ElevatedBioengineering (Basel, Switzerland) · 2024Article
- Biomimicking trilayer scaffolds with controlled estradiol release for uterine tissue regeneration.Exploration (Beijing, China) · 2024Article
- Review
- Recent Advances in Scaffolds for Guided Bone Regeneration.Biomimetics (Basel, Switzerland) · 2024Review
- Decellularized extracellular matrix biomaterials for regenerative therapies: Advances, challenges and clinical prospects.Bioactive materials · 2024Review
- A biomechanical assessment of tissue-engineered polymer neo-uteri after orthotopic implantation.F&S science · 2024Article
- Decellularisation and Characterisation of Porcine Pleura as Bioscaffolds in Tissue Engineering.Journal of tissue engineering and regenerative medicine · 2024Article
- Current status and challenges in uterine myometrial tissue engineering.Bioengineered · 2023Review
- Decellularization Techniques for Tissue Engineering: Towards Replicating Native Extracellular Matrix Architecture in Liver Regeneration.Journal of functional biomaterials · 2023Review
- New Solutions for Old Problems: How Reproductive Tissue Engineering Has Been Revolutionizing Reproductive Medicine.Annals of biomedical engineering · 2023Review
- Review
- Co-Culture of Mouse Blastocysts on A Human Recellularized Endometrial Scaffold: AnCell journal · 2023Article
- Effect of Pressure Conditions in Uterine Decellularization Using Hydrostatic Pressure on Structural Protein Preservation.Bioengineering (Basel, Switzerland) · 2023Article
- Differentiation of human endometrial mesenchymal cells to epithelial and stromal cells by seeding on the decellularized endometrial scaffold.In vitro cellular & developmental biology. Animal · 2023Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infertility caused by ovarian or tubal problems can be treated using In Vitro Fertilization and Embryo Transfer (IVF-ET); however, this is not possible for women with uterine loss and malformations that require uterine reconstruction for the treatment of their infertility. In this study, we are the first to report the usefulness of decellularized matrices as a scaffold for uterine reconstruction. Uterine tissues were extracted from Sprague Dawley (SD) rats and decellularized using either sodium dodecyl sulfate (SDS) or high hydrostatic pressure (HHP) at optimized conditions. Histological staining and quantitative analysis showed that both SDS and HHP methods effectively removed cells from the tissues with, specifically, a significant reduction of DNA contents for HHP constructs. HHP constructs highly retained the collagen content, the main component of extracellular matrices in uterine tissue, compared to SDS constructs and had similar content levels of collagen to the native tissue. The mechanical strength of the HHP constructs was similar to that of the native tissue, while that of the SDS constructs was significantly elevated. Transmission electron microscopy (TEM) revealed no apparent denaturation of collagen fibers in the HHP constructs compared to the SDS constructs. Transplantation of the decellularized tissues into rat uteri revealed the successful regeneration of the uterine tissues with a 3-layer structure 30 days after the transplantation. Moreover, a lot of epithelial gland tissue and Ki67 positive cells were detected. Immunohistochemical analyses showed that the regenerated tissues have a normal response to ovarian hormone for pregnancy. The subsequent pregnancy test after 30 days transplantation revealed successful pregnancy for both the SDS and HHP groups. These findings indicate that the decellularized matrix from the uterine tissue can be a potential scaffold for uterine regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.