Evidence map›Paper›PMID 25057925›Full record

ArticleShock (Augusta, Ga.)2014

Regulation of endothelial cell inflammation and lung polymorphonuclear lymphocyte infiltration by transglutaminase 2.

Kaiser M Bijli, Bryce G Kanter, Mohammad Minhajuddin, Antony Leonard, Lei Xu, Fabeha Fazal, Arshad Rahman

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Shock (Augusta, Ga.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. Article
  7. Autophagy inhibitor 3-methyladenine protects against endothelial cell barrier dysfunction in acute lung injury.American journal of physiology. Lung cellular and molecular physiology · 2018
    Article
  8. Article
  9. Article
  10. Phospholipase C-ε signaling mediates endothelial cell inflammation and barrier disruption in acute lung injury.American journal of physiology. Lung cellular and molecular physiology · 2016
    Article
  11. Regulation of allergic lung inflammation by endothelial cell transglutaminase 2.American journal of physiology. Lung cellular and molecular physiology · 2015
    Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Kaiser M BijliDepartments of *Pediatrics and †Biomedical Genetics, Lung Biology and Disease Program, University of Rochester School of Medicine and Dentistry, Rochester, New York.
Bryce G Kanter
Mohammad Minhajuddin
Antony Leonard
Lei Xu
Fabeha Fazal
Arshad Rahman
University of Rochester · US

Funding

VISUAL INDICES OF NEUROTOXICITYP30ES001247 · NIEHS · UNIVERSITY OF ROCHESTER · PI Martha Susiarjo · 1985 to 2026
$42.8M
G-protein-induced Signaling of PMN-mediated Lung InjuryR01HL067424 · NHLBI · UNIVERSITY OF ROCHESTER · PI RAHMAN, ARSHAD · 2002 to 2010
$2.3M
Regulation of Endothelial ICAM-1 and PMN-Mediated Lung Injury by Actin DynamicsR01HL096907 · NHLBI · UNIVERSITY OF ROCHESTER · PI FAZAL, FABEHA · 2010 to 2014
$1.5M
Regulation of Lung Authopagy and InflammationR01HL116632 · NHLBI · UNIVERSITY OF ROCHESTER · PI RAHMAN, ARSHAD · 2013 to 2016
$1.5M
NHLBI NIH HHS HL096907NHLBI NIH HHS HL116632NHLBI NIH HHS HL67424NHLBI NIH HHS R01 HL067424NHLBI NIH HHS R01 HL096907NHLBI NIH HHS R01 HL116632NIEHS NIH HHS ES-01247NIEHS NIH HHS P30 ES001247
6 · The paper itself

Abstract

We addressed the role of transglutaminase 2 (TG2), a calcium-dependent enzyme that catalyzes cross-linking of proteins, in the mechanism of endothelial cell (EC) inflammation and lung polymorphonuclear lymphocyte (PMN) infiltration. Exposure of EC to thrombin, a procoagulant and proinflammatory mediator, resulted in activation of the transcription factor nuclear factor κB (NF-κB) and its target genes, vascular cell adhesion molecule 1, monocyte chemotactic protein 1, and interleukin 6. RNAi knockdown of TG2 inhibited these responses. Analysis of NF-κB activation pathway showed that TG2 knockdown was associated with inhibition of thrombin-induced DNA binding as well as serine phosphorylation of RelA/p65, a crucial event that controls transcriptional capacity of the DNA-bound RelA/p65. These results implicate an important role for TG2 in mediating EC inflammation by promoting DNA-binding and transcriptional activity of RelA/p65. Because thrombin is released in high amounts during sepsis, and its concentration is elevated in plasma and lavage fluids of patients with acute respiratory distress syndrome, we determined the in vivo relevance of TG2 in a mouse model of sepsis-induced lung PMN recruitment. A marked reduction in NF-κB activation, adhesion molecule expression, and lung PMN sequestration was observed in TG2 knockout mice compared with wild-type mice exposed to endotoxemia. Together, these results identify TG2 as an important mediator of EC inflammation and lung PMN sequestration associated with intravascular coagulation and sepsis.

Indexed as

Gene Expression Regulation, EnzymologicAnimalsCell AdhesionCells, CulturedEndothelial CellsEndotoxemiaFibrosisGTP-Binding ProteinsHumansInflammationLungLymphocytesMiceMice, Inbred C57BLMice, KnockoutNeutrophilsGTP-Binding ProteinsNF-kappa BProtein Glutamine gamma Glutamyltransferase 2RELA protein, humanRela protein, mouseThrombinTranscription Factor RelATransglutaminases

Identifiers

PMID25057925
PMCPMC4236274
OpenAlexW2008349932

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.