ArticleLab on a chip2014
A microchip platform for interrogating tumor-macrophage paracrine signaling at the single-cell level.
Article in Lab on a chip, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 58 citations in OpenAlex.
- Nested Nanowell Arrays for High-Throughput Quantitative Analysis of Cytokines from Single Macrophages.ACS nano medicine · 2026Article
- Phenotypic plasticity and secretory heterogeneity in subpopulations derived from single cancer cell.Acta pharmaceutica Sinica. B · 2025Article
- Single Cell-Pair Proteomics for Decoding Immune-Cancer Cell Interactions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Profiling signaling mediators for cell-cell interactions and communications with microfluidics-based single-cell analysis tools.iScience · 2025Review
- Microfluidic Biochips for Single-Cell Isolation and Single-Cell Analysis of Multiomics and Exosomes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Antibodies, repertoires and microdevices in antibody discovery and characterization.Lab on a chip · 2024Review
- Thermal inkjet makes label-free single-cell proteomics accessible and easy.Frontiers in chemistry · 2024Article
- On-chip modeling of tumor evolution: Advances, challenges and opportunities.Materials today. Bio · 2023Review
- Deciphering Cell-Cell Interactions with Integrative Single-Cell Secretion Profiling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Single-cell sorting based on secreted products for functionally defined cell therapies.Microsystems & nanoengineering · 2022Review
- Measuring single-cell protein secretion in immunology: Technologies, advances, and applications.European journal of immunology · 2021Review
- Evaluation of cytotoxic T lymphocyte-mediated anticancer response against tumor interstitium-simulating physical barriers.Scientific reports · 2020Article
- Recent advances in microfluidic platforms for single-cell analysis in cancer biology, diagnosis and therapy.Trends in analytical chemistry : TRAC · 2019Article
- Dynamic secretome of bone marrow-derived stromal cells reveals a cardioprotective biochemical cocktail.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Multiplexed PCR-Free Detection of MicroRNAs in Single Cancer Cells Using a DNA-Barcoded Microtrough Array Chip.Micromachines · 2019Article
- Pushing the limits of detection for proteins secreted from single cells using quantum dots.The Analyst · 2019Article
- Review
- Longitudinal multiparameter assay of lymphocyte interactions from onset by microfluidic cell pairing and culture.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Single-cell analysis tools for drug discovery and development.Nature reviews. Drug discovery · 2016Review
- Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization.Sensors (Basel, Switzerland) · 2016Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
It is increasingly recognized that infiltrating immune cells contribute to the pathogenesis of a wide range of solid tumors. The paracrine signaling between the tumor and the immune cells alters the functional state of individual tumor cells and, correspondingly, the anticipated response to radiation or chemotherapies, which is of great importance to clinical oncology. Here we present a high-density microchip platform capable of measuring a panel of paracrine signals associated with heterotypic tumor-immune cell interactions in the single-cell, pair-wise manner. The device features a high-content cell capture array of 5000+ sub-nanoliter microchambers for the isolation of single and multi-cell combinations and a multi-plex antibody "barcode" array for multiplexed protein secretion analysis from each microchamber. In this work, we measured a panel of 16 proteins produced from individual glioma cells, individual macrophage cells and varying heterotypic multi-cell combinations of both on the same device. The results show changes of tumor cell functional phenotypes that cannot be explained by an additive effect from isolated single cells and, presumably, can be attributed to the paracrine signaling between macrophage and glioma cells. The protein correlation analysis reveals the key signaling nodes altered by tumor-macrophage communication. This platform enables the novel pair-wise interrogation of heterotypic cell-cell paracrine signaling at the individual cell level with an in-depth analysis of the changing functional phenotypes for different co-culture cell combinations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.