A complete molecular diagnostic procedure for applications in surveillance and subtyping of avian influenza virus.

Chun-Hsien Tseng, Hsiang-Jung Tsai, Chung-Ming Chang

Open access · hybridAbstract read

In one paragraph

Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it

0cells of the map it votes in

4citing papers in PubMed

0.9field-weighted citation impact, top 25% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 14 citations in OpenAlex.

Challenges for Precise Subtyping and Sequencing of a H5N1 Clade 2.3.4.4b Highly Pathogenic Avian Influenza Virus Isolated in Japan in the 2022-2023 Season Using Classical Serological and Molecular Methods.Viruses · 2023
Article
Detection of H5N1 High Pathogenicity Avian Influenza Viruses in Four Raptors and Two Geese in Japan in the Fall of 2022.Viruses · 2023
Article
Zoonotic Influenza and Human Health-Part 2: Clinical Features, Diagnosis, Treatment, and Prevention Strategies.Current infectious disease reports · 2018
Review
Advanced biosensors for detection of pathogens related to livestock and poultry.Veterinary research · 2017
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Chun-Hsien TsengAnimal Health Research Institute, Danshui, Taipei 25158, Taiwan.

Hsiang-Jung TsaiAnimal Health Research Institute, Danshui, Taipei 25158, Taiwan ; Graduate Institute of Veterinary Medicine, College of Bioresource and Agriculture, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 106, Taiwan.

Chung-Ming ChangResearch Center for Emerging Viral Infections, Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Kwei-Shan, Taoyuan 333, Taiwan.

Animal Health Research Institute · TWChang Gung University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionThe following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing.

methodThis study provides a specific and sensitive step-by-step procedure for efficient avian influenza identification of 16 hemagglutinin and 9 neuraminidase avian influenza subtypes. RESULT AND

conclusionThis diagnostic procedure may prove exceedingly useful for virological and ecological advancements in global avian influenza research.

Indexed as

AnimalsBenzothiazolesBirdsDiaminesGenome, ViralHemagglutinin Glycoproteins, Influenza VirusInfluenza in BirdsNeuraminidaseOrganic ChemicalsPathology, MolecularPolymerase Chain ReactionQuinolinesReverse Transcriptase Polymerase Chain ReactionSequence Analysis, DNABenzothiazolesDiamineshemagglutinin, avian influenza A virusHemagglutinin Glycoproteins, Influenza VirusNeuraminidaseOrganic ChemicalsQuinolinesSYBR Green I

Identifiers

PMID25057497

DOI10.1155/2014/653056

PMCPMC4098948

OpenAlexW2128175373

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.