ArticleGut pathogens2014
Detection, identification and quantification of Campylobacter jejuni, coli and lari in food matrices all at once using multiplex qPCR.
Article in Gut pathogens, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed.
- The Impact of Training Intervention on Levels of Indicator Bacteria and Prevalence of Selected Pathogens in Raw Milk From Smallholder Women Dairy Farmers in Central Ethiopia.Journal of food protection · 2025Article
- Using Wastewater Surveillance to Monitor Gastrointestinal Pathogen Infections in the State of Oklahoma.Microorganisms · 2023Article
- Diagnostic Investigation of 100 Cases of Abortion in Sheep in Uruguay: 2015-2021.Frontiers in veterinary science · 2022Article
- Determinants ofParasitology · 2021Article
- Characterization and Phylogenetic Analysis ofInternational journal of environmental research and public health · 2019Article
- Generation and characterization of a novel recombinant scFv antibody specific for Campylobacter jejuni.Applied microbiology and biotechnology · 2018Article
- A Comparative Evaluation Study of Growth Conditions for Culturing the Isolates of Campylobacter spp.Current microbiology · 2018Article
- Rapid Molecular Approach for Simultaneous Detection ofOsong public health and research perspectives · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThermotolerant Campylobacter jejuni, coli and lari are recognized as leading food-borne pathogens causing an acute bacterial enteritis worldwide. Due to narrow spectrum of their biochemical activity, it is very complicated to distinguish between individual species. For reliable risk assessment, proper incidence evaluation or swift sample analysis regarding individual species, a demand for simple and rapid method for their distinguishing is reasonable. In this study, we evaluated a reliable and simple approach for their simultaneous detection, species identification and quantification using multiplex qPCR.
resultsSpecies specific primers and hydrolysis probes are directed to hippuricase gene of C. jejuni, serine hydroxymethyltransferase gene of C. coli and peptidase T gene of C. lari. Efficiencies of reactions were 90.85% for C. jejuni, 96.97% for C. coli and 92.89% for C. lari. At 95.00% confidence level and when cut off is set to 38 cycles, limits of detection are in all cases under 10 genome copies per reaction which is very appreciated since it is known that infectious doses are very low.
conclusionsProposed assay was positively validated on different food matrices (chicken wing rinses, chicken juice and homogenized fried chicken strips). No inhibition of PCR reaction occurred. Assay was evaluated in accordance with MIQE handbook.
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