ArticleMolecular human reproduction2014
Sperm-specific post-acrosomal WW-domain binding protein (PAWP) does not cause Ca2+ release in mouse oocytes.
Article in Molecular human reproduction, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
24 citing papers in PubMed, 2 syntheses or guidelines pooled it, 64 citations in OpenAlex.
- Clinical Applications of Assisted Oocyte Activation in Couples with Various Reproductive Problems: A Systematic Review.Reproductive sciences (Thousand Oaks, Calif.) · 2024Pooled it
- Molecular Drivers of Developmental Arrest in the Human Preimplantation Embryo: A Systematic Review and Critical Analysis Leading to Mapping Future Research.International journal of molecular sciences · 2021Pooled it
- Genomic Insights into Fertilization: Tracing PLCZ1 Orthologs Across Amphibian Lineages.Genome biology and evolution · 2025Article
- Oocyte activation with phospholipase Cζ mRNA induces repetitive intracellular CaThe Journal of reproduction and development · 2024Article
- Sperm-Induced CaCells · 2023Review
- The Therapeutic and Diagnostic Potential of Phospholipase C Zeta, Oocyte Activation, and Calcium in Treating Human Infertility.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Ouabain-induced activation of phospholipase C zeta and its contributions to bovine sperm capacitation.Cell and tissue research · 2021Article
- Intracytoplasmic Sperm Injection in Cattle.Genes · 2021Review
- Sperm-oocyte interplay: an overview of spermatozoon's role in oocyte activation and current perspectives in diagnosis and fertility treatment.Cell & bioscience · 2021Review
- Diagnosis and Treatment of Male Infertility-Related Fertilization Failure.Journal of clinical medicine · 2020Review
- Increasing associations between defects in phospholipase C zeta and conditions of male infertility: not just ICSI failure?Journal of assisted reproduction and genetics · 2020Review
- Essential Role of Sperm-Specific PLC-Zeta in Egg Activation and Male Factor Infertility: An Update.Frontiers in cell and developmental biology · 2020Review
- Phospholipase C.Advances in experimental medicine and biology · 2020Review
- The eggstraordinary story of how life begins.Molecular reproduction and development · 2019Review
- "This is where it all started" - the pivotal role of PLCζ within the sophisticated process of mammalian reproduction: a systemic review.Basic and clinical andrology · 2017Article
- Sperm contributions to oocyte activation: more that meets the eye.Journal of assisted reproduction and genetics · 2016Review
- Homozygous mutation of PLCZ1 leads to defective human oocyte activation and infertility that is not rescued by the WW-binding protein PAWP.Human molecular genetics · 2016Article
- Signal transduction in mammalian oocytes during fertilization.Cell and tissue research · 2016Review
- Evaluation of PLCζ and PAWP Expression in Globozoospermic Individuals.Cell journal · 2016Article
- Essential Role of the EF-hand Domain in Targeting Sperm Phospholipase Cζ to Membrane Phosphatidylinositol 4,5-Bisphosphate (PIP2).The Journal of biological chemistry · 2015Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Mature mammalian oocytes undergo a prolonged series of cytoplasmic calcium (Ca(2+)) oscillations at fertilization that are the cause of oocyte activation. The Ca(2+) oscillations in mammalian oocytes are driven via inositol 1,4,5-trisphosphate (IP3) generation. Microinjection of the sperm-derived phospholipase C-zeta (PLCζ), which generates IP3, causes the same pattern of Ca(2+) oscillations as observed at mammalian fertilization and it is thought to be the physiological agent that triggers oocyte activation. However, another sperm-specific protein, 'post-acrosomal WW-domain binding protein' (PAWP), has also been reported to elicit activation when injected into mammalian oocytes, and to produce a Ca(2+) increase in frog oocytes. Here we have investigated whether PAWP can induce fertilization-like Ca(2+) oscillations in mouse oocytes. Recombinant mouse PAWP protein was found to be unable to hydrolyse phosphatidylinositol 4,5-bisphosphate in vitro and did not cause any detectable Ca(2+) release when microinjected into mouse oocytes. Microinjection with cRNA encoding either the untagged PAWP, or yellow fluorescent protein (YFP)-PAWP, or luciferase-PAWP fusion proteins all failed to trigger Ca(2+) increases in mouse oocytes. The lack of response in mouse oocytes was despite PAWP being robustly expressed at similar or higher concentrations than PLCζ, which successfully initiated Ca(2+) oscillations in every parallel control experiment. These data suggest that sperm-derived PAWP is not involved in triggering Ca(2+) oscillations at fertilization in mammalian oocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.