ArticleMolecular biology of the cell2014
Mad2, Bub3, and Mps1 regulate chromosome segregation and mitotic synchrony in Giardia intestinalis, a binucleate protist lacking an anaphase-promoting complex.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Multiple intermolecular interactions facilitate rapid evolution of essential genes.Nature ecology & evolution · 2023Article
- Microtubule organelles in Giardia.Advances in parasitology · 2020Review
- RNA-sequencing Profiles of Cell Cycle-Related Genes Upregulated during the G2-Phase in Giardia lamblia.The Korean journal of parasitology · 2019Article
- Myosin-independent cytokinesis inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Transcriptomics Indicates Active and Passive Metronidazole Resistance Mechanisms in Three SeminalFrontiers in microbiology · 2017Article
- The unconventional kinetoplastid kinetochore: from discovery toward functional understanding.Biochemical Society transactions · 2016Review
- Divergent Transcriptional Responses to Physiological and Xenobiotic Stress in Giardia duodenalis.Antimicrobial agents and chemotherapy · 2016Article
- Constitutive aneuploidy and genomic instability in the single-celled eukaryote Giardia intestinalis.MicrobiologyOpen · 2016Article
- Spindle assembly checkpoint proteins regulate and monitor meiotic synapsis in C. elegans.The Journal of cell biology · 2015Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The binucleate pathogen Giardia intestinalis is a highly divergent eukaryote with a semiopen mitosis, lacking an anaphase-promoting complex/cyclosome (APC/C) and many of the mitotic checkpoint complex (MCC) proteins. However, Giardia has some MCC components (Bub3, Mad2, and Mps1) and proteins from the cohesin system (Smc1 and Smc3). Mad2 localizes to the cytoplasm, but Bub3 and Mps1 are either located on chromosomes or in the cytoplasm, depending on the cell cycle stage. Depletion of Bub3, Mad2, or Mps1 resulted in a lowered mitotic index, errors in chromosome segregation (including lagging chromosomes), and abnormalities in spindle morphology. During interphase, MCC knockdown cells have an abnormal number of nuclei, either one nucleus usually on the left-hand side of the cell or two nuclei with one mislocalized. These results suggest that the minimal set of MCC proteins in Giardia play a major role in regulating many aspects of mitosis, including chromosome segregation, coordination of mitosis between the two nuclei, and subsequent nuclear positioning. The critical importance of MCC proteins in an organism that lacks their canonical target, the APC/C, suggests a broader role for these proteins and hints at new pathways to be discovered.
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