ArticleChembiochem : a European journal of chemical biology2014
A generalizable DNA-catalyzed approach to peptide-nucleic acid conjugation.
Article in Chembiochem : a European journal of chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Sequence-Dependent Acylation of Peptide Lysine Residues by DNAzymes.Chembiochem : a European journal of chemical biology · 2024Article
- Solution NMR Spectroscopy as a Tool to Study DNAzyme Structure and Function.Methods in molecular biology (Clifton, N.J.) · 2022Article
- DNAzymes for amine and peptide lysine acylation.Organic & biomolecular chemistry · 2021Article
- Molecular Features and Metal Ions That Influence 10-23 DNAzyme Activity.Molecules (Basel, Switzerland) · 2020Review
- Protein-Nucleic Acid Conjugation with Sterol Linkers Using Hedgehog Autoprocessing.Bioconjugate chemistry · 2019Article
- DNA-Catalyzed Introduction of Azide at Tyrosine for Peptide Modification.Angewandte Chemie (International ed. in English) · 2016Article
- Catalytic DNA: Scope, Applications, and Biochemistry of Deoxyribozymes.Trends in biochemical sciences · 2016Review
- Assessing histidine tags for recruiting deoxyribozymes to catalyze peptide and protein modification reactions.Organic & biomolecular chemistry · 2016Article
- DNA-Catalyzed Amide Hydrolysis.Journal of the American Chemical Society · 2016Article
- Identification of the Same Na(+)-Specific DNAzyme Motif from Two In Vitro Selections Under Different Conditions.Journal of molecular evolution · 2015Article
- Identification of Sequence-Selective Tyrosine Kinase Deoxyribozymes.Journal of molecular evolution · 2015Article
- Pursuing DNA catalysts for protein modification.Accounts of chemical research · 2015Article
- DNA-catalyzed lysine side chain modification.Angewandte Chemie (International ed. in English) · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We report DNA catalysts (deoxyribozymes) that join tyrosine-containing peptides to RNA and DNA in one step and without requiring protecting groups on either the peptide or the nucleic acid. Our previous efforts towards this goal required tethering the peptide to a DNA anchor oligonucleotide. Here, we established direct in vitro selection for deoxyribozymes that use untethered, free peptide substrates. This approach enables imposition of selection pressure via reduced peptide concentration and leads to preparatively useful lower apparent Km values of ∼100 μM peptide. Use of phosphorimidazolide (Imp) rather than triphosphate as the electrophile enables reactivity of either terminus (5' or 3') of both RNA and DNA. Our findings establish a generalizable means of joining unprotected peptide to nucleic acid in one step by using DNA catalysts identified by in vitro selection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.