Evidence map›Paper›PMID 25056922›Full record

ArticlePlant physiology2014

Symplastic phloem loading in poplar.

Cankui Zhang, Lu Han, Thomas L Slewinski, Jianlei Sun, Jing Zhang, Zeng-Yu Wang, Robert Turgeon

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
11.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 77 citations in OpenAlex.

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  15. Long-Distance Movement of mRNAs in Plants.Plants (Basel, Switzerland) · 2020
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Cankui ZhangDepartment of Plant Biology, Cornell University, Ithaca, New York 14853 (C.Z., T.L.S., J.S., J.Z., R.T.); andForage Improvement Division, The Noble Foundation, Ardmore, Oklahoma 73401 (L.H., Z-Y.W.) cz46@cornell.edu ert2@cornell.edu.ORCID http://orcid.org/0000-0002-9195-770X
Lu HanDepartment of Plant Biology, Cornell University, Ithaca, New York 14853 (C.Z., T.L.S., J.S., J.Z., R.T.); andForage Improvement Division, The Noble Foundation, Ardmore, Oklahoma 73401 (L.H., Z-Y.W.).
Thomas L SlewinskiDepartment of Plant Biology, Cornell University, Ithaca, New York 14853 (C.Z., T.L.S., J.S., J.Z., R.T.); andForage Improvement Division, The Noble Foundation, Ardmore, Oklahoma 73401 (L.H., Z-Y.W.).
Jianlei SunDepartment of Plant Biology, Cornell University, Ithaca, New York 14853 (C.Z., T.L.S., J.S., J.Z., R.T.); andForage Improvement Division, The Noble Foundation, Ardmore, Oklahoma 73401 (L.H., Z-Y.W.).
Jing ZhangDepartment of Plant Biology, Cornell University, Ithaca, New York 14853 (C.Z., T.L.S., J.S., J.Z., R.T.); andForage Improvement Division, The Noble Foundation, Ardmore, Oklahoma 73401 (L.H., Z-Y.W.).
Zeng-Yu WangDepartment of Plant Biology, Cornell University, Ithaca, New York 14853 (C.Z., T.L.S., J.S., J.Z., R.T.); andForage Improvement Division, The Noble Foundation, Ardmore, Oklahoma 73401 (L.H., Z-Y.W.).
Robert TurgeonDepartment of Plant Biology, Cornell University, Ithaca, New York 14853 (C.Z., T.L.S., J.S., J.Z., R.T.); andForage Improvement Division, The Noble Foundation, Ardmore, Oklahoma 73401 (L.H., Z-Y.W.) cz46@cornell.edu ert2@cornell.edu.
Cornell University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sap is driven through phloem sieve tubes by an osmotically generated pressure gradient between source and sink tissues. In many plants, source pressure results from thermodynamically active loading in which energy is used to transfer sucrose (Suc) from mesophyll cells to the phloem of leaf minor veins against a concentration gradient. However, in some species, almost all trees, correlative evidence suggests that sugar migrates passively through plasmodesmata from mesophyll cells into the sieve elements. The possibility of alternate loading mechanisms has important ramifications for the regulation of phloem transport and source-sink interactions. Here, we provide experimental evidence that, in gray poplar (Populus tremula × Populus alba), Suc enters the phloem through plasmodesmata. Transgenic plants were generated with yeast invertase in the cell walls to prevent Suc loading by this route. The constructs were driven either by the constitutive 35S promoter or the minor vein-specific galactinol synthase promoter. Transgenic plants grew at the same rate as the wild type without symptoms of loading inhibition, such as accumulation of carbohydrates or leaf chlorosis. Rates of photosynthesis were normal. In contrast, alfalfa (Medicago sativa) plants, which have limited numbers of plasmodesmata between mesophyll and phloem, displayed typical symptoms of loading inhibition when transformed with the same DNA constructs. The results are consistent with passive loading of Suc through plasmodesmata in poplar. We also noted defense-related symptoms in leaves of transgenic poplar when the plants were abruptly exposed to excessively high temperatures, adding to evidence that hexose is involved in triggering the hypersensitive response.

Indexed as

beta-FructofuranosidaseHot TemperatureMedicago sativaPhloemPlants, Genetically ModifiedPlasmodesmataPopulusbeta-Fructofuranosidase

Identifiers

PMID25056922
PMCPMC4149716
OpenAlexW1994185003

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.