ArticleNeural development2014
Pioneer midbrain longitudinal axons navigate using a balance of Netrin attraction and Slit repulsion.
Article in Neural development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Oculomotor axons use external and autocrine Slit signals for fasciculation, navigation, branching, and connectivity in mouse embryos.Research square · 2025Article
- Slit-independent guidance of longitudinal axons by Drosophila Robo3.Developmental biology · 2025Article
- Article
- Multiple roles for Pax2 in the embryonic mouse eye.Developmental biology · 2021Article
- Proteolytic cleavage of Slit by the Tolkin protease converts an axon repulsion cue to an axon growth cueDevelopment (Cambridge, England) · 2020Article
- Temporal regulation of axonal repulsion by alternative splicing of a conserved microexon in mammalianeLife · 2019Article
- Robo1 and 2 Repellent Receptors Cooperate to Guide Facial Neuron Cell Migration and Axon Projections in the Embryonic Mouse Hindbrain.Neuroscience · 2019Article
- A Subtle Network Mediating Axon Guidance: Intrinsic Dynamic Structure of Growth Cone, Attractive and Repulsive Molecular Cues, and the Intermediate Role of Signaling Pathways.Neural plasticity · 2019Review
- Motor neuron migration and positioning mechanisms: New roles for guidance cues.Seminars in cell & developmental biology · 2019Review
- Effects of Cyclic Tensile Strain on Oxidative Stress and the Function of Schwann Cells.BioMed research international · 2018Article
- Motor axons are guided to exit points in the spinal cord by Slit and Netrin signals.Developmental biology · 2017Article
- Article
- Synapsins Are Downstream Players of the BDNF-Mediated Axonal Growth.Molecular neurobiology · 2017Article
- Dscam1 Forms a Complex with Robo1 and the N-Terminal Fragment of Slit to Promote the Growth of Longitudinal Axons.PLoS biology · 2016Article
- Semaphorin 3C Released from a Biocompatible Hydrogel Guides and Promotes Axonal Growth of Rodent and Human Dopaminergic Neurons.Tissue engineering. Part A · 2016Article
- Netrin1-DCC-Mediated Attraction Guides Post-Crossing Commissural Axons in the Hindbrain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Subrepellent doses of Slit1 promote Netrin-1 chemotactic responses in subsets of axons.Neural development · 2015Article
- Motor neuron cell bodies are actively positioned by Slit/Robo repulsion and Netrin/DCC attraction.Developmental biology · 2015Article
- Plasticity for axolotl lens regeneration is associated with age-related changes in gene expression.Regeneration (Oxford, England) · 2014Article
- Clinical and Genetic Analysis of A Father-Son Duo with Monomelic Amyotrophy: Case Report.Annals of Indian Academy of NeurologyArticle
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundLongitudinal axons grow parallel to the embryonic midline to connect distant regions of the central nervous system. Previous studies suggested that repulsive midline signals guide pioneer longitudinal axons by blocking their entry into the floor plate; however, the role of midline attractants, and whether attractant signals may cooperate with repulsive signals, remains unclear. In this study we investigated the navigation of a set of pioneer longitudinal axons, the medial longitudinal fasciculus, in mouse embryos mutant for the Netrin/Deleted in Colorectal Cancer (DCC) attractants, and for Slit repellents, as well as the responses of explanted longitudinal axons in vitro.
resultsIn mutants for Netrin1 chemoattractant or DCC receptor signaling, longitudinal axons shifted away from the ventral midline, suggesting that Netrin1/DCC signals act attractively to pull axons ventrally. Analysis of mutants in the three Slit genes, including Slit1/2/3 triple mutants, suggest that concurrent repulsive Slit/Robo signals push pioneer axons away from the ventral midline. Combinations of mutations between the Netrin and Slit guidance systems provided genetic evidence that the attractive and repulsive signals balance against each other. This balance is demonstrated in vitro using explant culture, finding that the cues can act directly on longitudinal axons. The explants also reveal an unexpected synergy of Netrin1 and Slit2 that promotes outgrowth.
conclusionsThese results support a mechanism in which longitudinal trajectories are positioned by a push-pull balance between opposing Netrin and Slit signals. Our evidence suggests that longitudinal axons respond directly and simultaneously to both attractants and repellents, and that the combined signals constrain axons to grow longitudinally.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.