ArticleThe Journal of veterinary medical science2014
Establishment of ATP-based luciferase viability assay in 96-well plate for Trypanosoma congolense.
Article in The Journal of veterinary medical science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 30 citations in OpenAlex.
- In vitro trypanocidal activities and structure-activity relationships of ciprofloxacin analogs.Molecular diversity · 2024Article
- Article
- Two New Cytotoxic Sesquiterpene-Amino Acid Conjugates and a Coumarin-Glucoside fromMolecules (Basel, Switzerland) · 2023Article
- A Simple, Robust, and Affordable Bioluminescent Assay for Drug Screening Against Infective African Trypanosomes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- In Vitro and In Vivo Trypanocidal Efficacy of Synthesized Nitrofurantoin Analogs.Molecules (Basel, Switzerland) · 2021Article
- Six New Phenylpropanoid Derivatives from Chemically Converted Extract ofMolecules (Basel, Switzerland) · 2021Article
- Isolation and evaluation of trypanocidal activity of sesquiterpenoids, flavonoids, and lignans in Artemisia sieversiana collected in Mongolia.Journal of natural medicines · 2020Article
- Development of NanoLuc-PEST expressing Leishmania mexicana as a new drug discovery tool for axenic- and intramacrophage-based assays.PLoS neglected tropical diseases · 2018Article
- Lanostane triterpenoids from Fomitopsis officinalis and their trypanocidal activity.Journal of natural medicines · 2018Article
- Oral administration of azithromycin ameliorates trypanosomosis in Trypanosoma congolense-infected mice.Parasitology research · 2017Article
- The establishment of in vitro culture and drug screening systems for a newly isolated strain of Trypanosoma equiperdum.International journal for parasitology. Drugs and drug resistance · 2017Article
- Mycophenolic Acid and Its Derivatives as Potential Chemotherapeutic Agents Targeting Inosine Monophosphate Dehydrogenase in Trypanosoma congolense.Antimicrobial agents and chemotherapy · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Animal African trypanosomosis (AAT), caused by Trypanosoma congolense, is widespread throughout sub-Saharan Africa. There are significant concerns related to the current drugs available for the treatment of AAT due to their limited effectiveness across species and their adverse effects. Moreover, drug resistant trypanosomes have recently been reported in the field. High throughput screening (HTS) of large chemical compound library collections is a promising approach for identifying novel drug candidates. While HTS for Trypanozoon trypanosomes, T. brucei sspp. and T. evansi is well established, no assays have been developed for T. congolense. In the present study, the authors developed an ATP-based luciferase viability assay for T. congolense in a 96-well plate format. The calculated 50% inhibitory concentration (IC50) values for pentamidine and diminazene were 10-100 times higher in T. congolense than in T. brucei. This result suggests that the transporters for the 2 tested compounds differ between T. congolense and T. brucei. This assay could further be applied to screen novel chemical compounds for the treatment of AAT caused by T. congolense.
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