ArticleNucleic acids research2015
An efficient and sensitive method for preparing cDNA libraries from scarce biological samples.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Transcriptomic Profiling Identifies Key Genes and ERBB Signaling Pathway Associated with Aggressive Behavior in Muscovy Ducks (Animals : an open access journal from MDPI · 2026Article
- An absolute quantification atlas of small non-coding RNAs across diverse mammalian tissues and cell lines.Nature communications · 2026Article
- Integrated transcriptome and metabolome profiling ofFrontiers in genetics · 2022Article
- RIPiT-Seq: A tandem immunoprecipitation approach to reveal global binding landscape of multisubunit ribonucleoproteins.Methods in enzymology · 2021Article
- Extracellular microRNAs in human circulation are associated with miRISC complexes that are accessible to anti-AGO2 antibody and can bind target mimic oligonucleotides.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Decreasing miRNA sequencing bias using a single adapter and circularization approach.Genome biology · 2018Article
- Cloning and Identification of Recombinant Argonaute-Bound Small RNAs Using Next-Generation Sequencing.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Structure-seq2: sensitive and accurate genome-wide profiling of RNA structure in vivo.Nucleic acids research · 2017Article
- The in vivo genetic toolkit for studying expression and functions of Drosophila melanogaster microRNAs.RNA biology · 2017Review
- TargetLink, a new method for identifying the endogenous target set of a specific microRNA in intact living cells.RNA biology · 2017Article
- Plasma vesicle miRNAs for therapy response monitoring in Hodgkin lymphoma patients.JCI insight · 2016Article
- Toward reliable biomarker signatures in the age of liquid biopsies - how to standardize the small RNA-Seq workflow.Nucleic acids research · 2016Article
- Staufen Negatively Modulates MicroRNA Activity in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2016Article
- An optimized kit-free method for making strand-specific deep sequencing libraries from RNA fragments.Nucleic acids research · 2015Article
Corrections and comments
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Authors and funding
3 authors.
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Abstract
The preparation and high-throughput sequencing of cDNA libraries from samples of small RNA is a powerful tool to quantify known small RNAs (such as microRNAs) and to discover novel RNA species. Interest in identifying the small RNA repertoire present in tissues and in biofluids has grown substantially with the findings that small RNAs can serve as indicators of biological conditions and disease states. Here we describe a novel and straightforward method to clone cDNA libraries from small quantities of input RNA. This method permits the generation of cDNA libraries from sub-picogram quantities of RNA robustly, efficiently and reproducibly. We demonstrate that the method provides a significant improvement in sensitivity compared to previous cloning methods while maintaining reproducible identification of diverse small RNA species. This method should have widespread applications in a variety of contexts, including biomarker discovery from scarce samples of human tissue or body fluids.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.