ArticleNucleic acids research2014
Global absolute quantification reveals tight regulation of protein expression in single Xenopus eggs.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
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- Quantification of absolute transcription factor binding affinities in the native chromatin context using BANC-seq.Nature biotechnology · 2023Article
- Aberrant phase separation is a common killing strategy of positively charged peptides in biology and human disease.bioRxiv : the preprint server for biology · 2023Article
- Comparison of Different Label-Free Techniques for the Semi-Absolute Quantification of Protein Abundance.Proteomes · 2022Article
- Application of Domain- and Genotype-Specific Models to Infer Post-Transcriptional Regulation of Segmentation Gene Expression inLife (Basel, Switzerland) · 2021Article
- The molecular basis of coupling between poly(A)-tail length and translational efficiency.eLife · 2021Article
- Mass spectrometry based proteomics for developmental neurobiology in the amphibian Xenopus laevis.Current topics in developmental biology · 2021Review
- A GC-MS/Single-Cell Method to Evaluate Membrane Transporter Substrate Specificity and Signaling.Frontiers in molecular biosciences · 2021Article
- Transcriptomics and Proteomics Methods forCold Spring Harbor protocols · 2020Article
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- An integrated genome-wide multi-omics analysis of gene expression dynamics in the preimplantation mouse embryo.Scientific reports · 2019Article
- Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Single-cell proteomics in complex tissues using microprobe capillary electrophoresis mass spectrometry.Methods in enzymology · 2019Article
- Design and Application of Sensors for Chemical Cytometry.ACS chemical biology · 2018Review
- Integration of protein phosphorylation, acetylation, and methylation data sets to outline lung cancer signaling networks.Science signaling · 2018Article
- Quantitative Proteomics After Spinal Cord Injury (SCI) in a Regenerative and a Nonregenerative Stage in the FrogMolecular & cellular proteomics : MCP · 2018Article
- Quantitative Proteomics of Xenopus Embryos I, Sample Preparation.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Gibbs free energy as a measure of complexity correlates with time within C. elegans embryonic development.Journal of biological physics · 2017Article
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6 authors.
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Abstract
While recent developments in genomic sequencing technology have enabled comprehensive transcriptome analyses of single cells, single cell proteomics has thus far been restricted to targeted studies. Here, we perform global absolute protein quantification of fertilized Xenopus laevis eggs using mass spectrometry-based proteomics, quantifying over 5800 proteins in the largest single cell proteome characterized to date. Absolute protein amounts in single eggs are highly consistent, thus indicating a tight regulation of global protein abundance. Protein copy numbers in single eggs range from tens of thousands to ten trillion copies per cell. Comparison between the single-cell proteome and transcriptome reveal poor expression correlation. Finally, we identify 439 proteins that significantly change in abundance during early embryogenesis. Downregulated proteins include ribosomal proteins and upregulated proteins include basal transcription factors, among others. Many of these proteins do not show regulation at the transcript level. Altogether, our data reveal that the transcriptome is a poor indicator of the proteome and that protein levels are tightly controlled in X. laevis eggs.
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