Evidence map›Paper›PMID 25055193›Full record

ArticleStem cells and development2014

Derivation of mesenchymal stromal cells from canine induced pluripotent stem cells by inhibition of the TGFβ/activin signaling pathway.

Deanne J Whitworth, Jessica E Frith, Thomas J R Frith, Dmitry A Ovchinnikov, Justin J Cooper-White, Ernst J Wolvetang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells and development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Review
  6. Review
  7. Review
  8. Article
  9. Article
  10. Article
  11. Review
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Induced pluripotent stem cells in cartilage repair.World journal of orthopedics · 2016
    Article
  18. Article
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Deanne J Whitworth1 School of Veterinary Science, University of Queensland , Gatton, Queensland, Australia .
Jessica E Frith
Thomas J R Frith
Dmitry A Ovchinnikov
Justin J Cooper-White
Ernst J Wolvetang
University of Queensland · AUMaterials Science & Engineering · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study we have generated canine mesenchymal stromal cells (MSCs), also known as mesenchymal stem cells, from canine induced pluripotent stem cells (ciPSCs) by small-molecule inhibition of the transforming growth factor beta (TGFβ)/activin signaling pathway. These ciPSC-derived MSCs (ciPSC-MSCs) express the MSC markers CD73, CD90, CD105, STRO1, cPDGFRβ and cKDR, in addition to the pluripotency factors OCT4, NANOG and REX1. ciPSC-MSCs lack immunostaining for H3K27me3, suggesting that they possess two active X chromosomes. ciPSC-MSCs are highly proliferative and undergo robust differentiation along the osteo-, chondro- and adipogenic pathways, but do not form teratoma-like tissues in vitro. Of further significance for the translational potential of ciPSC-MSCs, we show that these cells can be encapsulated and maintained within injectable hydrogel matrices that, when functionalized with bound pentosan polysulfate, dramatically enhance chondrogenesis and inhibit osteogenesis. The ability to efficiently derive large numbers of highly proliferative canine MSCs from ciPSCs that can be incorporated into injectable, functionalized hydrogels that enhance their differentiation along a desired lineage constitutes an important milestone towards developing an effective MSC-based therapy for osteoarthritis in dogs, but equally provides a model system for assessing the efficacy and safety of analogous approaches for treating human degenerative joint diseases.

Indexed as

ChondrogenesisOsteogenesisSignal TransductionActivinsAnimalsAntigens, CDCells, CulturedChemokine CXCL12DogsGuanine Nucleotide Exchange FactorsHistonesInduced Pluripotent Stem CellsMesenchymal Stem CellsOctamer Transcription Factor-3Pentosan Sulfuric PolyesterReceptor, Platelet-Derived Growth Factor betaActivinsAntigens, CDChemokine CXCL12Guanine Nucleotide Exchange FactorsHistonesOctamer Transcription Factor-3Pentosan Sulfuric PolyesterReceptor, Platelet-Derived Growth Factor betaTransforming Growth Factor betaVascular Endothelial Growth Factor Receptor-2

Identifiers

PMID25055193
PMCPMC4267570
OpenAlexW2017345436

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.