ArticlePloS one2014
2-Butanol and butanone production in Saccharomyces cerevisiae through combination of a B12 dependent dehydratase and a secondary alcohol dehydrogenase using a TEV-based expression system.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 47 citations in OpenAlex.
- Direct Production of 2-Butanol from Glucose by RecombinantInternational journal of molecular sciences · 2026Article
- Process optimization for mass production of 2,3-butanediol by Bacillus subtilis CS13.Biotechnology for biofuels · 2021Article
- Rational design of a new mutant of tobacco etch virus protease in order to increase theResearch in pharmaceutical sciences · 2020Article
- A tight cold-inducible switch built by coupling thermosensitive transcriptional and proteolytic regulatory parts.Nucleic acids research · 2019Article
- Growth-coupled bioconversion of levulinic acid to butanone.Metabolic engineering · 2019Article
- Integrated Vacuum Stripping and Adsorption for the Efficient Recovery of (Biobased) 2-Butanol.Industrial & engineering chemistry research · 2019Article
- Microbial 2-butanol production withBiotechnology for biofuels · 2019Article
- Metabolic engineering ofBiotechnology for biofuels · 2017Article
- Growth inhibition of S. cerevisiae, B. subtilis, and E. coli by lignocellulosic and fermentation products.Applied microbiology and biotechnology · 2016Article
- Post-translational control of genetic circuits using Potyvirus proteases.Nucleic acids research · 2016Article
- Engineering Escherichia coli for Microbial Production of Butanone.Applied and environmental microbiology · 2016Article
- Increased availability of NADH in metabolically engineered baker's yeast improves transaminase-oxidoreductase coupled asymmetric whole-cell bioconversion.Microbial cell factories · 2016Article
- Engineering Bacillus licheniformis for the production of meso-2,3-butanediol.Biotechnology for biofuels · 2016Article
- Opportunities for Bio-Based Solvents Created as Petrochemical and Fuel Products Transition towards Renewable Resources.International journal of molecular sciences · 2015Review
- Metabolic Engineering of Klebsiella pneumoniae for the Production of 2-Butanone from Glucose.PloS one · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
2-Butanol and its chemical precursor butanone (methyl ethyl ketone--MEK) are chemicals with potential uses as biofuels and biocommodity chemicals. In order to produce 2-butanol, we have demonstrated the utility of using a TEV-protease based expression system to achieve equimolar expression of the individual subunits of the two protein complexes involved in the B12-dependent dehydratase step (from the pdu-operon of Lactobacillus reuteri), which catalyze the conversion of meso-2,3-butanediol to butanone. We have furthermore identified a NADH dependent secondary alcohol dehydrogenase (Sadh from Gordonia sp.) able to catalyze the subsequent conversion of butanone to 2-butanol. A final concentration of 4±0.2 mg/L 2-butanol and 2±0.1 mg/L of butanone was found. A key factor for the production of 2-butanol was the availability of NADH, which was achieved by growing cells lacking the GPD1 and GPD2 isogenes under anaerobic conditions.
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