ArticlePloS one2014
Modulating notochordal differentiation of human induced pluripotent stem cells using natural nucleus pulposus tissue matrix.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- The notochord: development, disease and stem cell-based modelling.Development (Cambridge, England) · 2026Review
- Engineering Intervertebral Disc Regeneration: Biomaterials, Cell Sources and Animal Models.Cell proliferation · 2025Review
- Article
- An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration.Bioactive materials · 2023Article
- Review
- New strategies for the treatment of intervertebral disc degeneration: cell, exosome, gene, and tissue engineering.American journal of translational research · 2022Review
- Development, Pathogenesis, and Regeneration of the Intervertebral Disc: Current and Future Insights Spanning Traditional to Omics Methods.Frontiers in cell and developmental biology · 2022Review
- Induction of notochordal differentiation of bone marrow mesenchymal‑derived stem cells via the stimulation of notochordal cell‑rich nucleus pulposus tissue.Molecular medicine reports · 2021Article
- IGF Signaling in Intervertebral Disc Health and Disease.Frontiers in cell and developmental biology · 2021Review
- Notochordal Cell-Based Treatment Strategies and Their Potential in Intervertebral Disc Regeneration.Frontiers in cell and developmental biology · 2021Review
- Intervertebral Disc Stem/Progenitor Cells: A Promising "Seed" for Intervertebral Disc Regeneration.Stem cells international · 2021Review
- Derivation of notochordal cells from human embryonic stem cells reveals unique regulatory networks by single cell-transcriptomics.Journal of cellular physiology · 2020Article
- Article
- Stem cell therapy in discogenic back pain.Journal of spine surgery (Hong Kong) · 2019Review
- Human iPSCs can be differentiated into notochordal cells that reduce intervertebral disc degeneration in a porcine model.Theranostics · 2019Article
- Intradiscal Injection of Induced Pluripotent Stem Cell-Derived Nucleus Pulposus-Like Cell-Seeded Polymeric Microspheres Promotes Rat Disc Regeneration.Stem cells international · 2019Article
- Development of the axial skeleton and intervertebral disc.Current topics in developmental biology · 2019Review
- Critical aspects and challenges for intervertebral disc repair and regeneration-Harnessing advances in tissue engineering.JOR spine · 2018Review
- Differentiation of human induced pluripotent stem cells into nucleus pulposus-like cells.Stem cell research & therapy · 2018Article
- Cell therapy for the degenerating intervertebral disc.Translational research : the journal of laboratory and clinical medicine · 2017Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human induced pluripotent stem cells (hiPSCs) can differentiate into notochordal cell (NC)-like cells when cultured in the presence of natural porcine nucleus pulposus (NP) tissue matrix. The method promises massive production of high-quality, functional cells to treat degenerative intervertebral discs (IVDs). Based on our previous work, we further examined the effect of cell-NP matrix contact and culture medium on the differentiation, and further assessed the functional differentiation ability of the generated NC-like. The study showed that direct contact between hiPSCs and NP matrix can promote the differentiation yield, whilst both the contact and non-contact cultures can generate functional NC-like cells. The generated NC-like cells are highly homogenous regarding the expression of notochordal marker genes. A culture medium containing a cocktail of growth factors (FGF, EGF, VEGF and IGF-1) also supported the notochordal differentiation in the presence of NP matrix. The NC-like cells showed excellent functional differentiation ability to generate NP-like tissue which was rich in aggrecan and collagen type II; and particularly, the proteoglycan to collagen content ratio was as high as 12.5-17.5 which represents a phenotype close to NP rather than hyaline cartilage. Collectively, the present study confirmed the effectiveness and flexibility of using natural NP tissue matrix to direct notochordal differentiation of hiPSCs, and the potential of using the generated NC-like cells for treating IVD degeneration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.